Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACEZDF_RS01565 Genome accession   NZ_CP169539
Coordinates   334120..335136 (+) Length   338 a.a.
NCBI ID   WP_005382774.1    Uniprot ID   A0AAP5Z0M3
Organism   Vibrio alginolyticus strain BR53     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329120..340136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEZDF_RS01550 (ACEZDF_01550) - 329551..330279 (-) 729 WP_005382781.1 redoxin family protein -
  ACEZDF_RS01555 (ACEZDF_01555) oxyR 330449..331360 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  ACEZDF_RS01560 (ACEZDF_01560) - 331414..333975 (-) 2562 WP_025768081.1 penicillin-binding protein 1A -
  ACEZDF_RS01565 (ACEZDF_01565) pilM 334120..335136 (+) 1017 WP_005382774.1 type IV pilus biogenesis protein PilM Machinery gene
  ACEZDF_RS01570 (ACEZDF_01570) pilN 335120..335695 (+) 576 WP_054579557.1 PilN domain-containing protein Machinery gene
  ACEZDF_RS01575 (ACEZDF_01575) pilO 335688..336281 (+) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEZDF_RS01580 (ACEZDF_01580) pilP 336271..336786 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  ACEZDF_RS01585 (ACEZDF_01585) pilQ 336812..338557 (+) 1746 WP_025768080.1 type IV pilus secretin PilQ Machinery gene
  ACEZDF_RS01590 (ACEZDF_01590) aroK 338745..339263 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37609.97 Da        Isoelectric Point: 7.2224

>NTDB_id=1051919 ACEZDF_RS01565 WP_005382774.1 334120..335136(+) (pilM) [Vibrio alginolyticus strain BR53]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGVNSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1051919 ACEZDF_RS01565 WP_005382774.1 334120..335136(+) (pilM) [Vibrio alginolyticus strain BR53]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGTAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment