Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACEZDF_RS01570 Genome accession   NZ_CP169539
Coordinates   335120..335695 (+) Length   191 a.a.
NCBI ID   WP_054579557.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain BR53     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330120..340695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEZDF_RS01555 (ACEZDF_01555) oxyR 330449..331360 (+) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  ACEZDF_RS01560 (ACEZDF_01560) - 331414..333975 (-) 2562 WP_025768081.1 penicillin-binding protein 1A -
  ACEZDF_RS01565 (ACEZDF_01565) pilM 334120..335136 (+) 1017 WP_005382774.1 type IV pilus biogenesis protein PilM Machinery gene
  ACEZDF_RS01570 (ACEZDF_01570) pilN 335120..335695 (+) 576 WP_054579557.1 PilN domain-containing protein Machinery gene
  ACEZDF_RS01575 (ACEZDF_01575) pilO 335688..336281 (+) 594 WP_017633532.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEZDF_RS01580 (ACEZDF_01580) pilP 336271..336786 (+) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  ACEZDF_RS01585 (ACEZDF_01585) pilQ 336812..338557 (+) 1746 WP_025768080.1 type IV pilus secretin PilQ Machinery gene
  ACEZDF_RS01590 (ACEZDF_01590) aroK 338745..339263 (+) 519 WP_005381319.1 shikimate kinase AroK -
  ACEZDF_RS01595 (ACEZDF_01595) aroB 339296..340396 (+) 1101 WP_005381318.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22361.92 Da        Isoelectric Point: 7.4658

>NTDB_id=1051920 ACEZDF_RS01570 WP_054579557.1 335120..335695(+) (pilN) [Vibrio alginolyticus strain BR53]
MLYRINLLPWRENQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQERLDYLTHYIAELDQRIEAMKIAELEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=1051920 ACEZDF_RS01570 WP_054579557.1 335120..335695(+) (pilN) [Vibrio alginolyticus strain BR53]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAATCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGAGC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCTAGAGCACAGC
AAAATCCTCGAACGTCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCTGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

63.277

92.67

0.586

Multiple sequence alignment