Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   O1Q80_RS08805 Genome accession   NZ_CP169368
Coordinates   1855934..1856455 (-) Length   173 a.a.
NCBI ID   WP_386687064.1    Uniprot ID   -
Organism   Lonepinella sp. MS14435     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1850934..1861455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q80_RS08785 (O1Q80_01752) - 1851207..1851476 (+) 270 WP_386694546.1 helix-turn-helix domain-containing protein -
  O1Q80_RS08790 (O1Q80_01753) ligA 1851811..1853829 (-) 2019 WP_386694549.1 NAD-dependent DNA ligase LigA -
  O1Q80_RS08795 (O1Q80_01754) zipA 1853913..1854917 (-) 1005 WP_386687060.1 cell division protein ZipA -
  O1Q80_RS08800 (O1Q80_01755) cysZ 1855064..1855915 (+) 852 WP_386687062.1 sulfate transporter CysZ -
  O1Q80_RS08805 (O1Q80_01756) pilF/pilF2 1855934..1856455 (-) 522 WP_386687064.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O1Q80_RS08810 (O1Q80_01757) - 1856499..1857635 (-) 1137 WP_386694552.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  O1Q80_RS08815 (O1Q80_01758) raiA 1857866..1858186 (-) 321 WP_386687068.1 ribosome-associated translation inhibitor RaiA -
  O1Q80_RS08820 (O1Q80_01759) metG 1858378..1860456 (-) 2079 WP_386694555.1 methionine--tRNA ligase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 19829.26 Da        Isoelectric Point: 5.6912

>NTDB_id=1051029 O1Q80_RS08805 WP_386687064.1 1855934..1856455(-) (pilF/pilF2) [Lonepinella sp. MS14435]
MKFIPHFILILFLTACVSQDVEIDFDQKPAAKARVELALGYLSQQDWEQAKQNLDKALSYAPDYYLVHSAFAYFYQQQGN
ITQARESYLKAIKLDDKQGDVHNNYGTFLCSQGEFQAAYQQFDLAVASPNYYRQADSYENKALCALSENNQAVYQQSKIA
LEKADPERAKKLR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1051029 O1Q80_RS08805 WP_386687064.1 1855934..1856455(-) (pilF/pilF2) [Lonepinella sp. MS14435]
ATGAAATTTATACCGCACTTTATACTTATTCTTTTTTTAACCGCTTGCGTTTCGCAAGATGTTGAAATAGATTTTGATCA
AAAGCCAGCCGCCAAAGCTCGAGTAGAACTCGCACTGGGCTATTTATCCCAACAAGATTGGGAACAAGCTAAACAAAATC
TGGATAAAGCCTTGAGTTATGCCCCTGATTACTATTTGGTTCATTCTGCCTTTGCTTATTTTTACCAACAACAAGGCAAT
ATTACACAAGCCCGTGAATCTTATTTGAAAGCCATTAAACTAGATGATAAACAAGGTGATGTGCATAATAATTATGGTAC
ATTTCTTTGTTCGCAAGGGGAGTTCCAAGCTGCCTACCAGCAATTTGATTTAGCAGTGGCAAGCCCGAATTATTATCGTC
AAGCAGATAGTTATGAAAATAAAGCCTTGTGTGCTTTATCGGAAAATAATCAGGCTGTTTATCAACAAAGTAAAATAGCG
TTAGAAAAAGCAGATCCTGAAAGGGCGAAGAAATTAAGATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(42-86)

Tetratricopeptide repeat (TPR_17)

(88-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

53.939

95.376

0.514
  pilF Glaesserella parasuis strain SC1401

46.471

98.266

0.457

Multiple sequence alignment