Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   O1Q82_RS08355 Genome accession   NZ_CP169367
Coordinates   1767835..1768356 (-) Length   173 a.a.
NCBI ID   WP_386687064.1    Uniprot ID   -
Organism   Lonepinella sp. MS14437     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1762835..1773356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q82_RS08335 (O1Q82_01665) - 1763054..1763494 (+) 441 WP_386687056.1 hypothetical protein -
  O1Q82_RS08340 (O1Q82_01666) ligA 1763712..1765730 (-) 2019 WP_386687058.1 NAD-dependent DNA ligase LigA -
  O1Q82_RS08345 (O1Q82_01667) zipA 1765814..1766818 (-) 1005 WP_386687060.1 cell division protein ZipA -
  O1Q82_RS08350 (O1Q82_01668) cysZ 1766965..1767816 (+) 852 WP_386687062.1 sulfate transporter CysZ -
  O1Q82_RS08355 (O1Q82_01669) pilF/pilF2 1767835..1768356 (-) 522 WP_386687064.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O1Q82_RS08360 (O1Q82_01670) - 1768399..1769535 (-) 1137 WP_386687066.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  O1Q82_RS08365 (O1Q82_01671) raiA 1769766..1770086 (-) 321 WP_386687068.1 ribosome-associated translation inhibitor RaiA -
  O1Q82_RS08370 (O1Q82_01672) metG 1770283..1772361 (-) 2079 WP_386687070.1 methionine--tRNA ligase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 19829.26 Da        Isoelectric Point: 5.6912

>NTDB_id=1051002 O1Q82_RS08355 WP_386687064.1 1767835..1768356(-) (pilF/pilF2) [Lonepinella sp. MS14437]
MKFIPHFILILFLTACVSQDVEIDFDQKPAAKARVELALGYLSQQDWEQAKQNLDKALSYAPDYYLVHSAFAYFYQQQGN
ITQARESYLKAIKLDDKQGDVHNNYGTFLCSQGEFQAAYQQFDLAVASPNYYRQADSYENKALCALSENNQAVYQQSKIA
LEKADPERAKKLR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=1051002 O1Q82_RS08355 WP_386687064.1 1767835..1768356(-) (pilF/pilF2) [Lonepinella sp. MS14437]
ATGAAATTTATACCGCACTTTATACTTATTCTTTTTTTAACCGCTTGCGTTTCGCAAGATGTTGAAATAGATTTTGATCA
AAAGCCAGCCGCCAAAGCTCGAGTAGAACTCGCACTGGGCTATTTATCCCAACAAGATTGGGAACAAGCTAAACAAAATC
TGGATAAAGCCTTGAGTTATGCCCCTGATTACTATTTGGTTCATTCCGCCTTTGCTTATTTTTACCAACAACAAGGCAAT
ATTACACAAGCCCGTGAATCTTATTTGAAAGCCATTAAACTAGATGATAAACAAGGCGATGTGCATAATAATTATGGTAC
ATTTCTTTGTTCGCAAGGGGAGTTCCAAGCTGCTTATCAGCAATTTGATTTAGCGGTGGCAAGCCCGAATTATTATCGTC
AAGCAGATAGTTATGAAAATAAAGCCTTGTGTGCTTTATCGGAAAATAATCAGGCGGTTTATCAACAAAGTAAAATAGCG
TTAGAAAAAGCAGATCCTGAAAGGGCGAAGAAATTAAGATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(42-86)

Tetratricopeptide repeat (TPR_17)

(88-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

53.939

95.376

0.514
  pilF Glaesserella parasuis strain SC1401

46.471

98.266

0.457

Multiple sequence alignment