Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ABJR18_RS04800 Genome accession   NZ_CP169359
Coordinates   1017699..1019078 (+) Length   459 a.a.
NCBI ID   WP_040080753.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain Mh184.20180323.PV.D     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1012699..1024078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJR18_RS04770 (ABJR18_000955) yacG 1012941..1013126 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  ABJR18_RS04775 (ABJR18_000956) coaE 1013108..1013743 (-) 636 WP_020831305.1 dephospho-CoA kinase -
  ABJR18_RS04780 (ABJR18_000957) - 1013779..1014435 (-) 657 WP_006248404.1 prepilin peptidase -
  ABJR18_RS04785 (ABJR18_000958) pilC 1014438..1015634 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  ABJR18_RS04790 (ABJR18_000959) pilB 1015618..1017018 (-) 1401 WP_040080755.1 GspE/PulE family protein Machinery gene
  ABJR18_RS04795 (ABJR18_000960) pilA 1017039..1017518 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  ABJR18_RS04800 (ABJR18_000961) radA 1017699..1019078 (+) 1380 WP_040080753.1 DNA repair protein RadA Machinery gene
  ABJR18_RS04805 (ABJR18_000962) - 1019133..1019363 (-) 231 WP_006248398.1 YgjV family protein -
  ABJR18_RS04810 (ABJR18_000963) folA 1019483..1019977 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  ABJR18_RS04815 (ABJR18_000964) proB 1020089..1021180 (+) 1092 WP_006251346.1 glutamate 5-kinase -
  ABJR18_RS04820 (ABJR18_000965) - 1021180..1021944 (+) 765 WP_006248395.1 TSUP family transporter -
  ABJR18_RS04825 (ABJR18_000966) nth 1021947..1022579 (+) 633 WP_006248394.1 endonuclease III -
  ABJR18_RS04830 (ABJR18_000967) - 1022776..1024026 (+) 1251 WP_040080752.1 multifunctional CCA addition/repair protein -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49759.39 Da        Isoelectric Point: 8.1850

>NTDB_id=1050926 ABJR18_RS04800 WP_040080753.1 1017699..1019078(+) (radA) [Mannheimia haemolytica strain Mh184.20180323.PV.D]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKEAAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANRLGLPTANLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDASL
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLKEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQDLVIFGEVG
LAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLSDALDILYNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=1050926 ABJR18_RS04800 WP_040080753.1 1017699..1019078(+) (radA) [Mannheimia haemolytica strain Mh184.20180323.PV.D]
ATGGCAAAAGCACCTAAAACCGCTTATGTTTGTAACGATTGCGGGGCTGAATATTCCCGTTGGATGGGGCAATGTAAGGA
ATGTAAGGCTTGGAATACCATCAGTGAAGTGCGGTTGATTTCCAGCAAAGAAGCGGCGAAATCTGACCGCTTTAGCGGCT
ATGCCGGTGAAACGTCAGGCAAAGTACAAACGCTGTCTGACATCAGCTTGCAGGAAGTGCCTCGCTTTAGCAGTGGCTTT
AACGAGCTGGATCGTGTACTCGGTGGCGGCGTAGTGCCGGGGTCAGCAATTCTGATCGGCGGTCACCCGGGGGCGGGGAA
AAGTACGTTATTACTGCAAGTAATGTGCGGTTTAGCCCAACATCTGCCAACGCTGTATGTCACCGGTGAAGAGTCGTTAC
AACAAGTTGCAATGCGGGCAAATCGCTTAGGCTTACCAACGGCAAATCTAAAAATGTTGTCTGAAACCTCTGTCGAGCAT
ATTTGCAATATTGCAGATCAAGAAAAGCCCAAAATTATGGTGATTGACTCGATTCAGGTGATGCACTTGTCGGATATTCA
GTCCTCACCCGGCAGTGTTTCACAAGTGCGTGAATGTGCATCTTTCCTTACCCGCTACGCCAAAACCCGCCAAGTGGCGA
TTATTATGGTTGGGCACGTCACTAAAGATGGCACGCTTGCCGGCCCGAAAGTGTTGGAACACGCCATCGATGCTTCCTTG
CTGTTAGAAGGCGAATCCGATTCCCGTTTCCGTACTTTGCGTAGCCAGAAAAATCGTTTTGGTGCCGTCAATGAATTGGG
CGTATTTGCGATGACCGAACAAGGGCTAAAAGAGGTTAAAAACCCATCTGCGATTTTTTTAAGCCGTAGCGAAGAGCAAA
CGCCGGGCAGTTCGGTGATGGTGTTATGGGAAGGAACACGCCCATTGTTGGTTGAAATTCAAGCCTTAGTCGATCACTCA
ATGCTGGCAAACCCTCGCCGTGTGGCGGTTGGCTTAGATCATAACCGTTTATCGCTATTACTTGCGGTGCTACACCGACA
CGGTGGCTTGCAGATGTCGGATCAAGATGTGTTTGTAAATGTGGTCGGTGGGGTGAAAGTCACGGAAACCAGTGCCGATT
TAGCCTTAATTTTGGCGTTAATTTCCAGCTTCCGTAACCGTCCATTGCCGCAAGATTTGGTGATTTTTGGGGAAGTCGGC
TTAGCAGGGGAAATCCGCCCGGTTACCAGTGGGCAAGAGCGGATCAGCGAAGCCGCAAAACACGGCTTTAAACGGGCGAT
AATTCCTCACGGCAATGTCCCGAAAAAGGCAATTAAAGATATGCAGGTGTTCACCGTGAAAAAACTCAGTGATGCGTTAG
ATATTCTGTATAATCTCTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)

AAA domain (AAA_25)

(84-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.465

99.346

0.481
  radA Streptococcus mitis NCTC 12261

44.273

98.911

0.438
  radA Streptococcus mitis SK321

44.053

98.911

0.436
  radA Streptococcus pneumoniae TIGR4

43.573

100

0.436
  radA Streptococcus pneumoniae D39

43.573

100

0.436
  radA Streptococcus pneumoniae Rx1

43.573

100

0.436
  radA Streptococcus pneumoniae R6

43.573

100

0.436

Multiple sequence alignment