Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   CF65_RS00355 Genome accession   NZ_CP007502
Coordinates   73922..74473 (+) Length   183 a.a.
NCBI ID   WP_025298068.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans HK1651     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 68922..79473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CF65_RS00340 (CF65_00093) - 69872..71236 (+) 1365 WP_005547462.1 L-serine ammonia-lyase -
  CF65_RS00345 (CF65_00096) ansB 71417..72466 (-) 1050 WP_005568022.1 L-asparaginase 2 -
  CF65_RS00350 (CF65_00099) - 72740..73900 (+) 1161 WP_155266098.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  CF65_RS00355 (CF65_00101) pilF/pilF2 73922..74473 (+) 552 WP_025298068.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CF65_RS00360 (CF65_00105) - 74617..75672 (+) 1056 WP_005568019.1 helix-turn-helix domain-containing protein -
  CF65_RS00365 (CF65_00106) ispG 75684..76787 (+) 1104 WP_005542474.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CF65_RS00370 (CF65_00107) hisS 76809..78086 (+) 1278 WP_005589329.1 histidine--tRNA ligase -
  CF65_RS00375 (CF65_00108) - 78097..78711 (+) 615 WP_025298069.1 YfgM family protein -
  CF65_RS00380 (CF65_00109) - 78764..79216 (+) 453 WP_005568016.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20697.48 Da        Isoelectric Point: 8.8869

>NTDB_id=105049 CF65_RS00355 WP_025298068.1 73922..74473(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans HK1651]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTTYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=105049 CF65_RS00355 WP_025298068.1 73922..74473(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans HK1651]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCACCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

46.471

92.896

0.432

Multiple sequence alignment