Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACEWE4_RS10025 Genome accession   NZ_CP169311
Coordinates   2042604..2043158 (-) Length   184 a.a.
NCBI ID   WP_006251773.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain Mh184.20240815.PV.SD     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2037604..2048158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWE4_RS10010 (ACEWE4_002003) sppA 2037682..2039538 (-) 1857 WP_006249718.1 signal peptide peptidase SppA -
  ACEWE4_RS10015 (ACEWE4_002004) - 2039646..2040209 (+) 564 WP_006249720.1 nitroreductase family protein -
  ACEWE4_RS10020 (ACEWE4_002005) - 2040280..2042604 (-) 2325 WP_006251774.1 Tex family protein -
  ACEWE4_RS10025 (ACEWE4_002006) pilF 2042604..2043158 (-) 555 WP_006251773.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACEWE4_RS10030 (ACEWE4_002007) - 2043378..2043629 (+) 252 WP_006251772.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ACEWE4_RS10035 (ACEWE4_002008) - 2043613..2043960 (+) 348 WP_006251771.1 helix-turn-helix domain-containing protein -
  ACEWE4_RS10040 (ACEWE4_002009) - 2044001..2045161 (-) 1161 WP_020831129.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACEWE4_RS10045 (ACEWE4_002010) - 2045421..2046317 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  ACEWE4_RS10050 (ACEWE4_002011) - 2046330..2047073 (+) 744 WP_006250122.1 metal ABC transporter ATP-binding protein -
  ACEWE4_RS10055 (ACEWE4_002012) - 2047130..2047939 (-) 810 WP_006250123.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20976.90 Da        Isoelectric Point: 7.0805

>NTDB_id=1050365 ACEWE4_RS10025 WP_006251773.1 2042604..2043158(-) (pilF) [Mannheimia haemolytica strain Mh184.20240815.PV.SD]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=1050365 ACEWE4_RS10025 WP_006251773.1 2042604..2043158(-) (pilF) [Mannheimia haemolytica strain Mh184.20240815.PV.SD]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCTTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCACCCTACTATCACCAAGCGGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446

Multiple sequence alignment