Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACEYW7_RS01730 Genome accession   NZ_CP169298
Coordinates   360476..361216 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain AMA205     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355476..366216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEYW7_RS01715 (ACEYW7_01715) ponA 356063..358618 (-) 2556 WP_000736683.1 penicillin-binding protein PBP1a -
  ACEYW7_RS01720 (ACEYW7_01720) pilM 358780..359838 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  ACEYW7_RS01725 (ACEYW7_01725) pilN 359838..360479 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  ACEYW7_RS01730 (ACEYW7_01730) pilO 360476..361216 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEYW7_RS01735 (ACEYW7_01735) pilP 361227..361754 (+) 528 WP_000695058.1 pilus assembly protein PilP Machinery gene
  ACEYW7_RS01740 (ACEYW7_01740) pilQ 361817..363982 (+) 2166 WP_001017038.1 type IV pilus secretin PilQ family protein Machinery gene
  ACEYW7_RS01745 (ACEYW7_01745) aroK 363994..364563 (+) 570 WP_000643712.1 shikimate kinase AroK -
  ACEYW7_RS01750 (ACEYW7_01750) aroB 364592..365674 (+) 1083 WP_001194002.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=1050303 ACEYW7_RS01730 WP_000076101.1 360476..361216(+) (pilO) [Acinetobacter baumannii strain AMA205]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=1050303 ACEYW7_RS01730 WP_000076101.1 360476..361216(+) (pilO) [Acinetobacter baumannii strain AMA205]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCAAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGAGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAGGATAACAAGTCTGATATTCCTGTACTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment