Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AKG93_RS15360 Genome accession   NZ_CP169261
Coordinates   3337399..3338415 (-) Length   338 a.a.
NCBI ID   WP_029789968.1    Uniprot ID   -
Organism   Vibrio harveyi strain VH2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3332399..3343415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKG93_RS15335 (AKG93_015335) aroK 3333255..3333773 (-) 519 WP_005451507.1 shikimate kinase AroK -
  AKG93_RS15340 (AKG93_015340) pilQ 3333963..3335714 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  AKG93_RS15345 (AKG93_015345) pilP 3335749..3336264 (-) 516 WP_045485666.1 pilus assembly protein PilP Machinery gene
  AKG93_RS15350 (AKG93_015350) pilO 3336254..3336847 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  AKG93_RS15355 (AKG93_015355) pilN 3336840..3337415 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  AKG93_RS15360 (AKG93_015360) pilM 3337399..3338415 (-) 1017 WP_029789968.1 type IV pilus assembly protein PilM Machinery gene
  AKG93_RS15365 (AKG93_015365) - 3338594..3341164 (+) 2571 WP_053052380.1 penicillin-binding protein 1A -
  AKG93_RS15370 (AKG93_015370) oxyR 3341231..3342139 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  AKG93_RS15375 (AKG93_015375) - 3342314..3343042 (+) 729 WP_053052381.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38023.40 Da        Isoelectric Point: 6.4206

>NTDB_id=1050054 AKG93_RS15360 WP_029789968.1 3337399..3338415(-) (pilM) [Vibrio harveyi strain VH2]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDGYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1050054 AKG93_RS15360 WP_029789968.1 3337399..3338415(-) (pilM) [Vibrio harveyi strain VH2]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACG
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACTGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(237-326)

Type IV pilus assembly protein PilM; (PilM_2)

(8-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment