Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACEVB2_RS07435 Genome accession   NZ_CP169223
Coordinates   1538007..1538552 (+) Length   181 a.a.
NCBI ID   WP_374091439.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain W24_252     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1533007..1543552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVB2_RS07415 (ACEVB2_07415) - 1534335..1534835 (+) 501 WP_250023345.1 lipopolysaccharide assembly protein LapB -
  ACEVB2_RS07420 (ACEVB2_07420) - 1534850..1534915 (+) 66 Protein_1427 hypothetical protein -
  ACEVB2_RS07425 (ACEVB2_07425) - 1535006..1536604 (+) 1599 WP_374091438.1 tetratricopeptide repeat protein -
  ACEVB2_RS07430 (ACEVB2_07430) - 1536784..1537968 (+) 1185 WP_010907446.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACEVB2_RS07435 (ACEVB2_07435) pilF/pilF2 1538007..1538552 (+) 546 WP_374091439.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACEVB2_RS07440 (ACEVB2_07440) - 1538675..1539631 (+) 957 WP_014391405.1 RodZ family helix-turn-helix domain-containing protein -
  ACEVB2_RS07445 (ACEVB2_07445) ispG 1539658..1540761 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACEVB2_RS07450 (ACEVB2_07450) hisS 1540791..1542062 (+) 1272 WP_324636799.1 histidine--tRNA ligase -
  ACEVB2_RS07455 (ACEVB2_07455) - 1542081..1542692 (+) 612 WP_083004649.1 YfgM family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20859.31 Da        Isoelectric Point: 7.8917

>NTDB_id=1049909 ACEVB2_RS07435 WP_374091439.1 1538007..1538552(+) (pilF/pilF2) [Pasteurella multocida subsp. gallicida strain W24_252]
MNRTFRFGIASIFSLFLSACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDTVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1049909 ACEVB2_RS07435 WP_374091439.1 1538007..1538552(+) (pilF/pilF2) [Pasteurella multocida subsp. gallicida strain W24_252]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACACTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459

Multiple sequence alignment