Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   R8803_RS22965 Genome accession   NZ_OY754392
Coordinates   4687414..4689612 (+) Length   732 a.a.
NCBI ID   WP_001325632.1    Uniprot ID   A7ZUF0
Organism   Escherichia coli isolate F719     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4682414..4694612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8803_RS22955 (FGAF719_44220) rhsC 4682655..4686839 (-) 4185 WP_038431248.1 rhs element protein RhsC -
  R8803_RS22960 (FGAF719_44230) rpmE 4686999..4687211 (-) 213 WP_000710769.1 50S ribosomal protein L31 -
  R8803_RS22965 (FGAF719_44240) priA 4687414..4689612 (+) 2199 WP_001325632.1 primosomal protein N' Machinery gene
  R8803_RS22970 (FGAF719_44250) cytR 4689768..4690793 (+) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  R8803_RS22975 (FGAF719_44260) ftsN 4690885..4691844 (+) 960 WP_000068834.1 cell division protein FtsN -
  R8803_RS22980 (FGAF719_44270) hslV 4691937..4692467 (+) 531 WP_000208243.1 ATP-dependent protease subunit HslV -
  R8803_RS22985 (FGAF719_44280) hslU 4692477..4693808 (+) 1332 WP_001293343.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81715.99 Da        Isoelectric Point: 8.9077

>NTDB_id=1046461 R8803_RS22965 WP_001325632.1 4687414..4689612(+) (priA) [Escherichia coli isolate F719]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPDVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=1046461 R8803_RS22965 WP_001325632.1 4687414..4689612(+) (priA) [Escherichia coli isolate F719]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTGTCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTGCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGGTTA
CTGCTCTGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGTCAGGCGGTGGATCTTAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCGCTGGCGGCGTTACGGCAAGGCAAAATCTGGCGCGACCAGGTCGCGGAACTTGAATTT
AATGATGCCGCGTTGCAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAGCGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CAGATACTTTTTCTGCCTGGCTGCTGGCGGGCGTTACCGGTTCCGGTAAAACGGAGGTTTATCTCAGTGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGGTTTAATGCCCCCGTGGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGGCTTTCGGCGTGGTTGAAAGCGAAAA
ATGGCGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGCGTGATTGTCATTGAT
GAAGAGCACGACAGCTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTGTATCGTGCGCATAG
CGAGCAAATCCCGATTATTCTTGGCTCCGCAACGCCCGCGCTGGAAACGTTGTGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGCCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCAGGTCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCAACAATCAGGTTATCCTCTTTCTTAATCG
TCGGGGTTTTGCGCCTGCGCTACTGTGCCACGACTGCGGCTGGATTGCCGAATGCCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGTCAGCGTCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTTGCGCCGCTGTTCCCTGATGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGGGCGCTGGAACAGCAACTGGCGGAAGTACATCGCGGCGGTGCGA
GGATTTTGATCGGCACACAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTGGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAACGTTTCGCCCAGCTTTACACCCAGGTCGCCGGTCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCACCATCCGGAACATCCTCTGTTGCAAACGTTGCTCTATAAAGGCT
ACGACGCCTTTGCCGAACAGGCGCTGGCTGAGCGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCGCTGTTCCTGCAACAACTGCGTAATCTGATCCTTTCCAGCCCGCTGGCAGA
CGACAAACTGTGGGTTCTCGGCCCGGTTCCGGCACTGGCACCTAAGCGTGGTGGCCGCTGGCGCTGGCAGATCTTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTTATTAATACAATACCGGATTCCCGCAAG
GTGAAATGGGTGTTGGATGTCGATCCCATCGAAGGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

DEAD/DEAH box helicase (DEAD)

(203-362)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZUF0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

45.047

100

0.454