Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEN9T_RS22935 Genome accession   NZ_CP168610
Coordinates   2998369..2999880 (+) Length   503 a.a.
NCBI ID   WP_092973412.1    Uniprot ID   -
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2993369..3004880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS22910 (ACEN9T_22905) - 2994585..2995568 (+) 984 WP_413707154.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACEN9T_RS22915 (ACEN9T_22910) - 2995585..2996169 (+) 585 WP_092973406.1 KdsC family phosphatase -
  ACEN9T_RS22920 (ACEN9T_22915) lptC 2996174..2996806 (+) 633 WP_092973408.1 LPS export ABC transporter periplasmic protein LptC -
  ACEN9T_RS22925 (ACEN9T_22920) lptA 2996866..2997453 (+) 588 WP_252691821.1 lipopolysaccharide transport periplasmic protein LptA -
  ACEN9T_RS22930 (ACEN9T_22925) lptB 2997484..2998272 (+) 789 WP_004633384.1 LPS export ABC transporter ATP-binding protein -
  ACEN9T_RS22935 (ACEN9T_22930) rpoN/rpoN1 2998369..2999880 (+) 1512 WP_092973412.1 RNA polymerase factor sigma-54 Machinery gene
  ACEN9T_RS22940 (ACEN9T_22935) hpf 2999931..3000284 (+) 354 WP_004633388.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACEN9T_RS22945 (ACEN9T_22940) ptsN 3000631..3001086 (+) 456 WP_012761097.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACEN9T_RS22950 (ACEN9T_22945) hprK 3001160..3002134 (+) 975 WP_004633393.1 HPr(Ser) kinase/phosphatase -
  ACEN9T_RS22955 (ACEN9T_22950) - 3002321..3002662 (+) 342 WP_027678303.1 PsiF family protein -
  ACEN9T_RS22960 (ACEN9T_22955) rapZ 3002757..3003650 (+) 894 WP_027678304.1 RNase adapter RapZ -
  ACEN9T_RS22965 (ACEN9T_22960) - 3003755..3004408 (+) 654 WP_092973414.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55697.01 Da        Isoelectric Point: 4.8537

>NTDB_id=1044621 ACEN9T_RS22935 WP_092973412.1 2998369..2999880(+) (rpoN/rpoN1) [Ralstonia sp. Ralssp110 strain CT11-110]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSNSATPLPTE
SPPPVPNGADSGDSSENSYENGHDSGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLK
LSMRDKGLVIFLIESLDDEGYLSGTLDEILADLPAELEVELDELQATLRMLQSFDPPGVGARSAAECLSLQLHRLDSPAK
ALALTIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDV
MPRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADT
LGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVV
ARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=1044621 ACEN9T_RS22935 WP_092973412.1 2998369..2999880(+) (rpoN/rpoN1) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGAAACAGTCGCTCCAGCTCCGCCTCTCTCAGCATCTCGCGCTTACGCCGCAGTTGCAGCAATCGATCCGACTGCTGCA
GTTGTCGACCATCGAACTGCAGCAGGAAGTTGAGCAGGCCTTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACCTCTGCGCGCATTGGCGCAGACGGCTCCGTGAACGCGCTGCAAAGCAACAGCGCCACACCCCTGCCCACCGAA
TCACCCCCACCCGTACCCAACGGTGCCGACAGCGGCGACAGCTCGGAAAACAGCTACGAAAACGGTCACGACAGCGGCGC
CGATTTCGACAGCGATTACGGCTCCGACCGCTCCGACTGGAGCCTTGACGACTTCGCCCGCAAACCGCAATCCGACGAAG
ACGACCGCGCCCCGCTGCAGTTGCGTGAGGCCGAGCAAAGTCTGCGCGAATTCCTCATGGAACAACTCGCGCCGCTCAAG
CTGTCCATGCGAGACAAGGGCCTCGTCATCTTCCTGATCGAATCCCTCGACGACGAAGGCTACCTCTCTGGCACGCTCGA
CGAAATCCTCGCCGATCTGCCTGCCGAACTTGAAGTCGAACTCGACGAACTGCAAGCCACGCTGCGCATGCTGCAGAGCT
TCGACCCGCCTGGCGTCGGCGCGCGTTCAGCTGCTGAATGCCTGTCACTGCAATTGCATCGGCTCGATTCGCCCGCGAAG
GCGCTGGCGCTCACCATCGTCAATCAGCATCTCGAACTGCTGGCCGCGCGAGACTACACACGCCTGAAGAAAGCGCTGTC
CGTCGACGAGCCCGCGCTGAAGGCAGCGCATGAGCTGATTCGTTCGCTGGCACCGTTTCCGGGTCATGCATTCGGGCGTG
CTGAAGCGGATTTCGTGGTGCCCGACGTGATCGTGCGCAAGACCAGCGCAGGCTGGATGGCGCAACTGAACCCCGACGTC
ATGCCGCGCCTGCGCATCAACGACATGTACGCGCAAATCCTGCGCAGCAGCCGCGGTGAATCGGGCGCCGCCAATCTGCA
GCAGAAGCTGCAGGAAGCACGTTGGCTGATCAAGAACATTCAGCAGCGCTTCGATACGATCCTGCGTGTCTCGCAAGCCA
TTGTCGAGCGTCAAAAGAGCTTCTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACA
CTGGGTCTACACGAGTCCACGATCTCCCGTGTGACGACCAACAAATACATGGCGACCCCGATGGGGACGTTCGAGTTGAA
GTACTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAAC
TTGTAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTG
GCCCGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

93.439

100

0.934

Multiple sequence alignment