Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACEN3H_RS01665 Genome accession   NZ_CP168231
Coordinates   349069..349848 (+) Length   259 a.a.
NCBI ID   WP_373358436.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain 2024CK-01095     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 344069..354848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3H_RS01650 (ACEN3H_01650) ponA 344650..347211 (-) 2562 WP_062850224.1 penicillin-binding protein PBP1a -
  ACEN3H_RS01655 (ACEN3H_01655) pilM 347373..348431 (+) 1059 WP_373358435.1 pilus assembly protein PilM Machinery gene
  ACEN3H_RS01660 (ACEN3H_01660) pilN 348431..349072 (+) 642 WP_016143661.1 PilN domain-containing protein Machinery gene
  ACEN3H_RS01665 (ACEN3H_01665) pilO 349069..349848 (+) 780 WP_373358436.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACEN3H_RS01670 (ACEN3H_01670) pilP 349860..350387 (+) 528 WP_373358437.1 pilus assembly protein PilP Machinery gene
  ACEN3H_RS01675 (ACEN3H_01675) pilQ 350450..352615 (+) 2166 WP_016143658.1 type IV pilus secretin PilQ Machinery gene
  ACEN3H_RS01680 (ACEN3H_01680) aroK 352627..353196 (+) 570 WP_016142884.1 shikimate kinase AroK -
  ACEN3H_RS01685 (ACEN3H_01685) aroB 353225..354307 (+) 1083 WP_373358438.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29378.33 Da        Isoelectric Point: 6.0348

>NTDB_id=1043298 ACEN3H_RS01665 WP_373358436.1 349069..349848(+) (pilO) [Acinetobacter lactucae strain 2024CK-01095]
MSPHELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDSKSDIPVLNYSIKAKTYRYMGTGENQNNNDPQGGNGETQA
NTSDSHNVNIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=1043298 ACEN3H_RS01665 WP_373358436.1 349069..349848(+) (pilO) [Acinetobacter lactucae strain 2024CK-01095]
ATGAGTCCTCATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAATACACTCGATATGAACAGCTATGGTAGTTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCCTTGGGCTATTTTGTTGCTATTCAGCCAAAGCTCCAAGCAATTGATAATGCTCAGGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGTAATTTGCAGCAATATCAGCTTCAACTTCAAGAAAT
GCAAGCCAACTTCAACCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CAGGAGTGAATTCTGGTCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAGGAAATCTTTATTGAACAGCCG
ATTACGATTGAGGCAACAGGCGATTATCACGCTTTCGGTGCTTTCGTGAGCAGTATTGCGGCATTACCGCGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATAGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGCACTGGGGAAAACCAAAATAATAATGACCCACAAGGGGGAAATGGTGAGACGCAAGCC
AACACCTCCGATAGCCATAATGTAAATATTCCTTCTATCAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

88.839

86.486

0.768
  comO Acinetobacter baylyi ADP1

69.388

94.595

0.656

Multiple sequence alignment