Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACDZ04_RS08165 Genome accession   NZ_CP168017
Coordinates   1688564..1689250 (-) Length   228 a.a.
NCBI ID   WP_001794049.1    Uniprot ID   A0A0E1X4U4
Organism   Staphylococcus aureus strain sa230502_barcode25     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1683564..1694250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDZ04_RS08145 (ACDZ04_08145) rpsT 1684478..1684729 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  ACDZ04_RS08150 (ACDZ04_08150) holA 1684774..1685748 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  ACDZ04_RS08155 (ACDZ04_08155) comEC 1685805..1688006 (-) 2202 WP_000967506.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACDZ04_RS08160 (ACDZ04_08160) - 1688011..1688472 (-) 462 WP_000439692.1 ComE operon protein 2 -
  ACDZ04_RS08165 (ACDZ04_08165) comEA 1688564..1689250 (-) 687 WP_001794049.1 ComEA family DNA-binding protein Machinery gene
  ACDZ04_RS08170 (ACDZ04_08170) - 1689290..1690006 (-) 717 WP_000084835.1 class I SAM-dependent DNA methyltransferase -
  ACDZ04_RS08175 (ACDZ04_08175) rsfS 1690009..1690362 (-) 354 WP_001088020.1 ribosome silencing factor -
  ACDZ04_RS08180 (ACDZ04_08180) yqeK 1690363..1690947 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACDZ04_RS08185 (ACDZ04_08185) nadD 1690937..1691506 (-) 570 WP_000822938.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  ACDZ04_RS08190 (ACDZ04_08190) yhbY 1691509..1691799 (-) 291 WP_000955232.1 ribosome assembly RNA-binding protein YhbY -
  ACDZ04_RS08195 (ACDZ04_08195) aroE 1691803..1692609 (-) 807 WP_000666750.1 shikimate dehydrogenase -
  ACDZ04_RS08200 (ACDZ04_08200) yqeH 1692623..1693723 (-) 1101 WP_001280134.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25766.38 Da        Isoelectric Point: 9.3383

>NTDB_id=1041290 ACDZ04_RS08165 WP_001794049.1 1688564..1689250(-) (comEA) [Staphylococcus aureus strain sa230502_barcode25]
MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLNSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1041290 ACDZ04_RS08165 WP_001794049.1 1688564..1689250(-) (comEA) [Staphylococcus aureus strain sa230502_barcode25]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAGCTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
TTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTAGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGCAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAATTCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E1X4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

95.595

99.561

0.952
  comEA Staphylococcus aureus N315

95.595

99.561

0.952

Multiple sequence alignment