Detailed information NTG1041200

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACDZ05_RS07155
• Genome accession: NZ_CP168013
• Coordinates: 1519590..1520087 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain sa230711_barcode73
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1514590..1525087

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACDZ05_RS07130 (ACDZ05_07130)	gcvPB	1514752..1516224 (-)	1473	WP_000202184.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
ACDZ05_RS07135 (ACDZ05_07135)	gcvPA	1516217..1517563 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
ACDZ05_RS07140 (ACDZ05_07140)	gcvT	1517583..1518674 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
ACDZ05_RS07145 (ACDZ05_07145)	-	1518833..1519357 (-)	525	WP_001015120.1	shikimate kinase	-
ACDZ05_RS07150 (ACDZ05_07150)	-	1519347..1519493 (-)	147	WP_001789879.1	hypothetical protein	-
ACDZ05_RS07155 (ACDZ05_07155)	comGF	1519590..1520087 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACDZ05_RS07160 (ACDZ05_07160)	comGE	1520005..1520304 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
ACDZ05_RS07165 (ACDZ05_07165)	comGD	1520291..1520737 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACDZ05_RS07170 (ACDZ05_07170)	comGC	1520715..1521025 (-)	311	Protein_1405	competence type IV pilus major pilin ComGC	-
ACDZ05_RS07175 (ACDZ05_07175)	comGB	1521039..1522109 (-)	1071	WP_000776425.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACDZ05_RS07180 (ACDZ05_07180)	comGA	1522081..1523055 (-)	975	WP_031872164.1	competence type IV pilus ATPase ComGA	Machinery gene
ACDZ05_RS07185 (ACDZ05_07185)	-	1523107..1523730 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
ACDZ05_RS07190 (ACDZ05_07190)	-	1523727..1524056 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
ACDZ05_RS07195 (ACDZ05_07195)	-	1524056..1525042 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=1041200 ACDZ05_RS07155 WP_001825340.1 1519590..1520087(-) (comGF) [Staphylococcus aureus strain sa230711_barcode73]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1041200 ACDZ05_RS07155 WP_001825340.1 1519590..1520087(-) (comGF) [Staphylococcus aureus strain sa230711_barcode73]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97