Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   KJP45_RS07345 Genome accession   NZ_OX419578
Coordinates   1395793..1397892 (-) Length   699 a.a.
NCBI ID   WP_213414440.1    Uniprot ID   -
Organism   Bacillus subtilis isolate NRS6128     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1390793..1402892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP45_RS07315 (NRS6128_07260) spo0E 1390848..1391105 (+) 258 WP_003232480.1 aspartyl-phosphate phosphatase Spo0E -
  KJP45_RS07320 (NRS6128_07265) - 1391191..1391622 (+) 432 WP_003232479.1 hypothetical protein -
  KJP45_RS07325 (NRS6128_07270) kinD 1391650..1393170 (-) 1521 WP_080477770.1 sporulation kinase KinD -
  KJP45_RS07330 (NRS6128_07275) mhqR 1393363..1393800 (+) 438 WP_003232475.1 MarR family transcriptional regulator MhqR -
  KJP45_RS07335 (NRS6128_07280) motB 1393840..1394625 (-) 786 WP_003232473.1 flagellar motor protein MotB -
  KJP45_RS07340 (NRS6128_07285) motA 1394597..1395409 (-) 813 WP_003238984.1 flagellar motor stator protein MotA -
  KJP45_RS07345 (NRS6128_07290) clpE 1395793..1397892 (-) 2100 WP_213414440.1 ATP-dependent Clp protease ATP-binding subunit Regulator
  KJP45_RS07350 (NRS6128_07295) - 1398257..1399300 (+) 1044 WP_021479408.1 membrane protein -
  KJP45_RS07355 (NRS6128_07300) queC 1399613..1400272 (+) 660 WP_021479407.1 7-cyano-7-deazaguanine synthase QueC -
  KJP45_RS07360 (NRS6128_07305) queD 1400265..1400714 (+) 450 WP_003232462.1 6-carboxytetrahydropterin synthase QueD -
  KJP45_RS07365 (NRS6128_07310) queE 1400707..1401438 (+) 732 WP_003232460.1 7-carboxy-7-deazaguanine synthase QueE -
  KJP45_RS07370 (NRS6128_07315) queF 1401456..1401953 (+) 498 WP_213414441.1 preQ(1) synthase -
  KJP45_RS07375 (NRS6128_07320) ykvN 1402503..1402859 (-) 357 WP_015715792.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 77852.95 Da        Isoelectric Point: 5.1811

>NTDB_id=1041196 KJP45_RS07345 WP_213414440.1 1395793..1397892(-) (clpE) [Bacillus subtilis isolate NRS6128]
MRCQHCHQNEATIRLNMQINSVHKQMVLCETCYNELTRKPSMSMGPQSFGFPFEQAFQPKEHSAAKQSGKKGLLDELAQN
ITNGAKAGLIDPVIGRDDEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVA
NTGIRGQFEERMKQLITELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAAL
ERRFQPVMVQEPSIEQAILILQGIKDKYEAYHGITFSDEAIKACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLLIDELN
DEDAAERLTAIEAEKTKALEDENYELAAKLRDEELALEKKLNSSSAHTAVTVEAEHIQEIVEQKTGIPVGKLQADEQTKM
KELEAKLHERVIGQEAAVQKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDAIIRLDMSEYME
KHAVSKIIGSPPGYVGHEEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVIIMTSN
AGAGEKQTKVGFQSDDSVIEEQTLIDSLSMFFKPEFLNRFDSIIEFRPLEKEHLVKIVSLLLGELEETLAERGISLNVTD
EAKEKIAELGYHPSFGARPLRRTIQEWVEDEMTDLLLDNGEITSFHVILEDDKIKVRAK

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=1041196 KJP45_RS07345 WP_213414440.1 1395793..1397892(-) (clpE) [Bacillus subtilis isolate NRS6128]
ATGCGTTGTCAACATTGTCATCAAAACGAGGCGACGATTCGCCTTAACATGCAAATAAATTCCGTTCATAAACAGATGGT
TCTTTGTGAAACTTGCTATAACGAACTGACCCGTAAACCTTCAATGAGTATGGGTCCTCAATCTTTCGGATTTCCGTTTG
AACAGGCATTCCAGCCGAAAGAACACAGCGCAGCAAAACAAAGCGGAAAAAAAGGGTTGCTTGATGAGCTGGCTCAAAAT
ATTACAAACGGTGCTAAAGCCGGTCTCATTGATCCCGTCATCGGCCGTGATGATGAAGTGGCGCGAGTGATCGAAATTCT
AAACCGCCGCAACAAAAACAATCCGGTTCTTATTGGTGAGCCGGGTGTGGGGAAAACTGCCATCGCTGAAGGGCTCGCTT
TAAAAATTGCTGAAGGCGATGTTCCAAACAAACTGAAAAACAAAGAGCTATACTTGCTTGATGTTGCATCCCTTGTTGCA
AATACAGGGATCAGAGGCCAATTTGAGGAGAGAATGAAACAGCTGATCACTGAGCTGAAGGAACGAAAAAATGTCATTCT
GTTCATTGATGAAATTCACCTTCTCGTCGGCGCAGGCTCTGCAGAAGGATCAATGGACGCCGGCAACATTCTCAAACCGG
CCCTAGCCAGAGGCGAACTGCAAGTCATTGGCGCGACAACACTGAAAGAATATCGTCAAATCGAAAAAGATGCCGCGCTG
GAAAGACGTTTTCAGCCTGTCATGGTGCAGGAGCCTTCAATTGAACAGGCTATCCTCATTCTGCAAGGGATTAAAGACAA
ATACGAGGCATACCATGGCATAACATTCAGTGATGAAGCAATCAAAGCGTGTGTCACTTTATCATCCCGCTACATTCAGG
ACAGACACCTGCCGGATAAAGCAATTGATTTATTAGATGAAGCAGGTTCAAAAGCCAACCTGTTAATTGATGAACTGAAT
GATGAGGATGCCGCTGAACGCTTAACTGCAATTGAAGCCGAAAAAACAAAAGCCCTGGAAGATGAAAATTACGAACTGGC
GGCAAAACTCCGTGATGAAGAACTCGCATTGGAGAAAAAACTGAACAGCTCCTCCGCTCATACCGCTGTCACTGTGGAAG
CTGAGCACATTCAGGAAATTGTTGAACAAAAAACAGGCATCCCTGTCGGCAAACTGCAGGCAGACGAACAAACGAAAATG
AAAGAACTCGAAGCAAAACTTCATGAACGCGTGATTGGACAAGAAGCCGCTGTTCAAAAAGTGGCAAAGGCGGTAAGACG
AAGCCGCGCCGGTTTAAAATCCAAAAACAGACCAGTCGGCTCCTTCCTCTTCGTCGGTCCTACCGGCGTAGGGAAAACAG
AGCTTTCTAAAACACTGGCAGATGAATTATTCGGCACAAAAGACGCTATTATCCGACTCGATATGAGCGAATACATGGAG
AAACACGCCGTATCTAAAATTATCGGTTCACCGCCTGGATATGTCGGCCATGAGGAAGCTGGACAATTAACTGAGAAAGT
GCGCCGCAATCCTTACAGCATTGTGTTGCTGGATGAGATTGAAAAAGCACACCCAGACGTTCAGCATATGTTCCTGCAAA
TTATGGAAGATGGCCGTCTGACAGACAGCCAAGGCAGAACCGTAAGCTTCAAAGACACTGTGATCATCATGACAAGTAAT
GCGGGTGCTGGTGAGAAACAAACGAAAGTCGGTTTCCAATCAGATGACAGTGTCATCGAAGAACAAACATTAATTGATTC
ACTGAGCATGTTCTTTAAACCTGAGTTCCTCAACCGTTTTGACAGCATTATTGAGTTCCGCCCATTGGAAAAAGAACATC
TTGTCAAAATCGTCAGCCTTCTTCTTGGAGAACTTGAAGAAACATTGGCGGAACGGGGCATTAGCTTGAATGTGACAGAT
GAAGCGAAAGAAAAAATCGCTGAGCTGGGCTACCACCCTTCATTCGGTGCACGTCCGCTTAGAAGAACCATCCAAGAATG
GGTTGAGGATGAAATGACCGATCTGCTGCTTGATAATGGCGAGATCACAAGTTTTCACGTGATTTTAGAAGATGATAAAA
TCAAAGTGCGAGCGAAATAA

Domains

Predicted by InterProScan.

ATPase family associated with various cellular activities (AAA) (AAA)

(116-247)

AAA lid domain (AAA_lid_9)

(253-352)

AAA domain (Cdc48 subfamily) (AAA_2)

(437-603)

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(609-679)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.528

100

0.601
  clpC Lactococcus lactis subsp. cremoris KW2

56.148

100

0.588
  clpE Streptococcus pneumoniae TIGR4

54.646

100

0.564
  clpE Streptococcus pneumoniae Rx1

54.646

100

0.564
  clpE Streptococcus pneumoniae D39

54.646

100

0.564
  clpE Streptococcus pneumoniae R6

54.646

100

0.564
  clpC Bacillus subtilis subsp. subtilis str. 168

53.701

90.844

0.488
  clpC Streptococcus pneumoniae Rx1

45.579

93.848

0.428
  clpC Streptococcus pneumoniae D39

45.579

93.848

0.428
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

47.368

89.7

0.425
  clpC Streptococcus thermophilus LMD-9

45.122

93.848

0.423
  clpC Streptococcus thermophilus LMG 18311

44.665

93.848

0.419
  clpC Streptococcus pneumoniae TIGR4

46.142

90.844

0.419
  clpC Streptococcus mutans UA159

46.055

88.841

0.409
  clpA Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

41.914

86.695

0.363