Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACDW34_RS03170 Genome accession   NZ_CP167896
Coordinates   657975..658775 (+) Length   266 a.a.
NCBI ID   WP_372403984.1    Uniprot ID   -
Organism   Acinetobacter piscicola strain p38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 652975..663775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW34_RS03140 (ACDW34_03140) - 653311..653688 (+) 378 WP_130074096.1 hypothetical protein -
  ACDW34_RS03145 (ACDW34_03145) - 653780..655069 (+) 1290 WP_372403982.1 glycosyltransferase family 4 protein -
  ACDW34_RS03150 (ACDW34_03150) - 655099..655650 (+) 552 WP_180045224.1 phosphatase PAP2 family protein -
  ACDW34_RS03155 (ACDW34_03155) iscX 655774..655974 (+) 201 WP_130074099.1 Fe-S cluster assembly protein IscX -
  ACDW34_RS03160 (ACDW34_03160) ndk 656094..656525 (+) 432 WP_016166212.1 nucleoside-diphosphate kinase -
  ACDW34_RS03165 (ACDW34_03165) rlmN 656695..657930 (+) 1236 WP_372403983.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACDW34_RS03170 (ACDW34_03170) pilF 657975..658775 (+) 801 WP_372403984.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACDW34_RS03175 (ACDW34_03175) - 658766..659557 (+) 792 WP_130074102.1 helix-turn-helix domain-containing protein -
  ACDW34_RS03180 (ACDW34_03180) ispG 659581..660702 (+) 1122 WP_130074103.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACDW34_RS03185 (ACDW34_03185) hisS 660706..662001 (+) 1296 WP_130074104.1 histidine--tRNA ligase -
  ACDW34_RS03190 (ACDW34_03190) - 662030..662731 (+) 702 WP_174493321.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30138.31 Da        Isoelectric Point: 8.7419

>NTDB_id=1040161 ACDW34_RS03170 WP_372403984.1 657975..658775(+) (pilF) [Acinetobacter piscicola strain p38]
MYKKLITVAAISCAVVLTACETTQTGSVLKKDPEKAVKVRTQLAAEYIRTHDLDAAKRSLDQALEIDSGDSTANMMMGVL
LQQEGSKVSMDKADGYFKRAISRDPDNAQARNNYGTYLYLMKRYNDAIEQLQFAGASLGYDQRYRALENLGKVYMIVGDK
ENAEKTFKQTLQVNRDSVISLIELSELLYLKQQTAQASQLYEQFVRLVGQKNQDSRALWIGIRIARANGDQMGSQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=1040161 ACDW34_RS03170 WP_372403984.1 657975..658775(+) (pilF) [Acinetobacter piscicola strain p38]
ATGTACAAAAAATTGATCACAGTCGCTGCAATCAGTTGTGCTGTGGTCTTGACGGCTTGTGAAACGACACAAACAGGGTC
AGTTTTAAAAAAAGATCCTGAAAAAGCAGTCAAAGTACGTACTCAATTGGCTGCTGAATATATCCGAACGCATGATTTAG
ATGCCGCAAAACGTTCACTTGATCAAGCCTTAGAAATTGATTCAGGTGATTCGACTGCCAATATGATGATGGGGGTTTTA
CTGCAACAAGAAGGCAGTAAAGTCAGCATGGATAAAGCGGATGGTTATTTTAAACGTGCTATTTCCCGTGATCCAGACAA
TGCACAAGCCCGTAATAATTATGGGACTTACTTATATTTGATGAAGCGTTATAATGACGCCATTGAACAACTGCAGTTTG
CAGGTGCGAGTTTAGGCTATGACCAACGCTATAGAGCTTTGGAAAATCTCGGTAAAGTCTATATGATTGTAGGCGATAAA
GAGAATGCTGAAAAAACGTTCAAGCAAACGCTACAAGTCAATCGAGATTCTGTTATATCTTTAATCGAGTTGTCAGAACT
ATTATATTTAAAACAGCAAACCGCTCAAGCCAGTCAACTGTATGAGCAGTTTGTGCGTTTAGTGGGTCAGAAAAATCAAG
ACTCAAGAGCGCTTTGGATCGGAATTCGTATCGCGAGAGCGAATGGTGATCAGATGGGGTCGCAAGTATTGGTCAATCAA
TTACGCGCACTTTTTCCTGAAAGCCAAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_17)

(97-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

71.103

98.872

0.703
  pilF Acinetobacter baylyi ADP1

65.399

98.872

0.647

Multiple sequence alignment