Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ACCO40_RS05820 Genome accession   NZ_CP167793
Coordinates   1145545..1149249 (+) Length   1234 a.a.
NCBI ID   WP_372402619.1    Uniprot ID   -
Organism   Bacillus spizizenii strain SHT-15     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1140545..1154249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCO40_RS05805 (ACCO40_05805) - 1140988..1141317 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  ACCO40_RS05810 (ACCO40_05810) - 1141437..1141874 (-) 438 WP_114150733.1 ferritin family protein -
  ACCO40_RS05815 (ACCO40_05815) addB 1142058..1145558 (+) 3501 WP_114150732.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACCO40_RS05820 (ACCO40_05820) addA 1145545..1149249 (+) 3705 WP_372402619.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACCO40_RS05825 (ACCO40_05825) sbcD 1149321..1150496 (+) 1176 WP_041904943.1 exonuclease subunit SbcD -
  ACCO40_RS05830 (ACCO40_05830) sbcC 1150493..1153885 (+) 3393 WP_372402620.1 exonuclease subunit SbcC -
  ACCO40_RS05835 (ACCO40_05835) hlpB 1153899..1154201 (+) 303 WP_014113305.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141355.67 Da        Isoelectric Point: 5.0720

>NTDB_id=1039400 ACCO40_RS05820 WP_372402619.1 1145545..1149249(+) (addA) [Bacillus spizizenii strain SHT-15]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDISEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLGQLDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAEDETGERQPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDKTETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENDAAMHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIRKGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKNAIDIEDIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQVAKTNVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1039400 ACCO40_RS05820 WP_372402619.1 1145545..1149249(+) (addA) [Bacillus spizizenii strain SHT-15]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
AACACTTCATTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTATTTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAAGGCGAAAAGGCG
TTTTTTGAACTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCACGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATATATCGGAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCAAAGGAGAAGCTTCAGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGGGCAGCTTGATGAACT
GATTCAGCATCAGGATGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GAGACGAGTTTGATCCTGCACTCCTTGAAGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACAAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGACATGAAGCCTGTTATTGAAACGCTCGTACA
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGGATGAAACAGGTGAGCGCCAGCCGAGCGAGGCGGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAGGAAACAGGCAACCTGTTTATGGTAGGTGATGTCAAACAGTCCATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACAGGACGGAAAATTGACTTGAATAAAAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATAAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTCCGCTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCAATTGTCGGAGCGGATGAAAACGAGCTCTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGGAAACGT
TCTACGGACATCTTCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGTGC
GCGCCAATATGAATCGACGGCATTTCGAGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAG
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACGAAGTATATTCATCCCCAATTGCGCATCAGCTACCCGACGCTGCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCTCAAACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATCGGACCGGCTCTTGCCAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGATGCGGCTATGCATGCTGATATATCAGGACACCCGGCCCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAACGGAAGAAAAAAGCGAGCGCTTAGAGGCGATCCGCAAAGGTGAACCAGTTCC
CGGCTCGTTTGCATTTGATGAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCGCATCACGAGGTCACGCAAATTCGTA
CAAAACAGTCGGTTTCTGAGATTAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTTACCCATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAAACGCTATTGATATAGAAGATATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAACCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAGTTGCAAAAACAAATGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.353

100

0.964

Multiple sequence alignment