Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ACCO40_RS05815 Genome accession   NZ_CP167793
Coordinates   1142058..1145558 (+) Length   1166 a.a.
NCBI ID   WP_114150732.1    Uniprot ID   -
Organism   Bacillus spizizenii strain SHT-15     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1137058..1150558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCO40_RS05795 (ACCO40_05795) - 1137909..1139111 (-) 1203 WP_061188316.1 MFS transporter -
  ACCO40_RS05800 (ACCO40_05800) - 1139225..1140952 (+) 1728 WP_372402618.1 ABC transporter substrate-binding protein -
  ACCO40_RS05805 (ACCO40_05805) - 1140988..1141317 (-) 330 WP_041520913.1 four-helix bundle copper-binding protein -
  ACCO40_RS05810 (ACCO40_05810) - 1141437..1141874 (-) 438 WP_114150733.1 ferritin family protein -
  ACCO40_RS05815 (ACCO40_05815) addB 1142058..1145558 (+) 3501 WP_114150732.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACCO40_RS05820 (ACCO40_05820) addA 1145545..1149249 (+) 3705 WP_372402619.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACCO40_RS05825 (ACCO40_05825) sbcD 1149321..1150496 (+) 1176 WP_041904943.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134762.79 Da        Isoelectric Point: 5.4749

>NTDB_id=1039399 ACCO40_RS05815 WP_114150732.1 1142058..1145558(+) (addB) [Bacillus spizizenii strain SHT-15]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPFAEDIKGAHMYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTQTPELAHLEAQYEARPAIPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDEEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGIELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=1039399 ACCO40_RS05815 WP_114150732.1 1142058..1145558(+) (addB) [Bacillus spizizenii strain SHT-15]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTTCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGACAAAAGCGGATTTACCGCACAGGTTGAACGGATGCTGACAGAATTTAAGCGTTACT
GCCTGGAACCGGAAGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCAATTCTGTATCAGCAGATGGAAAAAAGCCTTGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATTCCGTTTGCGGAAGATATAAAAGGCGCGCATATGTATGTGGACGGCTTTTATCAATTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCACATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACGTATTATCGCCTGCATCAGAAGGCGAA
GGAACTGAATCTGGACATTACCTATAAAGAGCTAAGCGGGACTGAGCGGCATACGCAGACGCCGGAACTGGCACATCTAG
AGGCGCAGTATGAAGCGCGACCGGCCATCCCGTACGCAGAAAAACAAGAGGCTTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAACTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCTTCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAAACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAAACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAGGAAGACGGCAGAATTATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGAAGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTATTTGTCGGCAATATGGATTTATCCAGAATGTATGGCACCTCCTGCACCTTTGTGTTGGGGGCAAACGACGGCGTTC
TGCCTGCACGCCCCGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGGTTGGGATTGAGCTTTCC
TCAGGTGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCCGATGCGGAAGGAAAAACGCTTTTGCCATCGATTGTCATTAAACGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGTCTGTTGACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTTATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCAAGCCAGCTCAGATTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGTAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCATCACACGGGCTGCATCTAAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTAAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTTGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAACTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCGAAAGGCCTGCTCCTAAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTATCTTGA
TTTATCAATTACTCATTCAGCTGACTGGCTCGGAATGAGGGCGACGCCTGCCGGAGTGCTATATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGCGATCAGGAAGCGGTTCGCCTCATGGATACGACCCTTCAAGAGGGACGGTCGAATATCATCAACGCGGGCTT
GAAGAAAGACGGCTCTCTCAGATCGGATTCAGCGGCAGTCGGCGAGAAGGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACTGACGGACGCGTATCGATTGAGCCGTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAGTCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGATAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

98.113

100

0.981

Multiple sequence alignment