Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACDJ02_RS03900 Genome accession   NZ_CP167777
Coordinates   794744..795496 (+) Length   250 a.a.
NCBI ID   WP_004994852.1    Uniprot ID   A0A3G9FKH4
Organism   Acinetobacter ursingii strain E5131942     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 789744..800496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDJ02_RS03875 (ACDJ02_03875) - 790622..791899 (+) 1278 WP_151831590.1 glycosyltransferase family 1 protein -
  ACDJ02_RS03880 (ACDJ02_03880) - 791929..792480 (+) 552 WP_004992665.1 phosphatase PAP2 family protein -
  ACDJ02_RS03885 (ACDJ02_03885) iscX 792585..792785 (+) 201 WP_049174904.1 Fe-S cluster assembly protein IscX -
  ACDJ02_RS03890 (ACDJ02_03890) ndk 792897..793328 (+) 432 WP_004994861.1 nucleoside-diphosphate kinase -
  ACDJ02_RS03895 (ACDJ02_03895) rlmN 793450..794676 (+) 1227 WP_151831589.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACDJ02_RS03900 (ACDJ02_03900) pilF 794744..795496 (+) 753 WP_004994852.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACDJ02_RS03905 (ACDJ02_03905) - 795487..796269 (+) 783 WP_151831588.1 RodZ domain-containing protein -
  ACDJ02_RS03910 (ACDJ02_03910) ispG 796294..797412 (+) 1119 WP_004992682.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ACDJ02_RS03915 (ACDJ02_03915) hisS 797416..798708 (+) 1293 WP_004992685.1 histidine--tRNA ligase -
  ACDJ02_RS03920 (ACDJ02_03920) - 798738..799439 (+) 702 WP_004992686.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28394.23 Da        Isoelectric Point: 9.3924

>NTDB_id=1039313 ACDJ02_RS03900 WP_004994852.1 794744..795496(+) (pilF) [Acinetobacter ursingii strain E5131942]
MALLLTGCQSQKTTKDPKKAVQVRTQLAAEYIKSGDLDAAKRALDQALEVNSRDSMANMMMGVLLQQEGSATNLEKADTY
FKRAISEEPTNAQARNNYGTYLYQLKRYNDAIEQFTIAGATLGYDQRYRALENLGRIYVLLGDRVNAEKSFKQALQANRD
SYVSMLELSEIFYLQQQMPAATQMYEQYVRTVGQTNQGARALWVGIRVARANGDRMGMQALINQLRALFPDSSEYKRYLQ
LQYSTEAVWK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=1039313 ACDJ02_RS03900 WP_004994852.1 794744..795496(+) (pilF) [Acinetobacter ursingii strain E5131942]
ATGGCGTTGCTGTTAACGGGATGCCAATCTCAAAAAACAACCAAAGACCCTAAAAAAGCGGTGCAGGTCCGTACACAACT
TGCAGCTGAATATATTAAGTCTGGTGATCTGGATGCTGCTAAACGCGCATTGGATCAGGCTTTGGAAGTGAACTCGCGTG
ACTCTATGGCCAATATGATGATGGGTGTACTCTTACAACAAGAAGGTAGTGCAACAAATCTCGAGAAAGCAGACACGTAT
TTTAAACGTGCAATCTCGGAAGAGCCTACCAATGCACAAGCTCGTAATAATTATGGTACGTACTTATACCAATTGAAACG
TTATAATGACGCGATTGAACAATTTACGATTGCAGGGGCAACACTTGGGTATGATCAGCGTTATCGTGCCCTTGAAAATC
TGGGGCGTATCTACGTATTGCTTGGCGATCGTGTGAATGCTGAAAAGTCATTCAAACAAGCTTTACAGGCAAACCGCGAC
TCTTATGTATCTATGTTAGAGTTGTCAGAGATTTTTTATTTGCAACAGCAAATGCCAGCGGCGACTCAAATGTATGAACA
ATATGTGCGTACAGTGGGGCAAACCAATCAGGGTGCACGTGCGCTTTGGGTGGGGATTCGTGTTGCACGAGCCAATGGTG
ATCGAATGGGAATGCAGGCACTGATCAATCAACTGCGTGCTTTATTCCCAGATAGTTCAGAATATAAACGTTATTTGCAA
TTACAGTATAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(81-113)

Tetratricopeptide repeat (TPR_7)

(130-160)

Tetratricopeptide repeat (TPR_8)

(23-52)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G9FKH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

81.275

100

0.816
  pilF Acinetobacter baylyi ADP1

77.108

99.6

0.768

Multiple sequence alignment