Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   ACB352_RS02640 Genome accession   NZ_CP167009
Coordinates   514098..515423 (+) Length   441 a.a.
NCBI ID   WP_159316975.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain Isolate 22     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509098..520423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB352_RS02615 (ACB352_02615) - 509868..510509 (+) 642 WP_002983671.1 response regulator transcription factor -
  ACB352_RS02620 (ACB352_02620) - 510546..511946 (+) 1401 WP_021340868.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  ACB352_RS02625 (ACB352_02625) - 511946..512323 (+) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  ACB352_RS02630 (ACB352_02630) cysK 512341..513282 (-) 942 WP_011285046.1 cysteine synthase A -
  ACB352_RS02635 (ACB352_02635) - 513410..514042 (-) 633 WP_002988970.1 YigZ family protein -
  ACB352_RS02640 (ACB352_02640) comFA/cflA 514098..515423 (+) 1326 WP_159316975.1 DEAD/DEAH box helicase Machinery gene
  ACB352_RS02645 (ACB352_02645) - 515395..516060 (+) 666 WP_002983685.1 ComF family protein -
  ACB352_RS02650 (ACB352_02650) hpf 516140..516688 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49822.35 Da        Isoelectric Point: 10.0009

>NTDB_id=1037309 ACB352_RS02640 WP_159316975.1 514098..515423(+) (comFA/cflA) [Streptococcus pyogenes strain Isolate 22]
MESIENSYGRLLLESQLPDLAKQLAQPLKSVVISRGKMICQRCHYQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFNNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1037309 ACB352_RS02640 WP_159316975.1 514098..515423(+) (comFA/cflA) [Streptococcus pyogenes strain Isolate 22]
ATGGAGTCTATTGAAAATAGTTATGGACGATTGTTGCTAGAAAGCCAGTTACCAGATTTAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAATGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTAATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae D39

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae R6

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae TIGR4

53.938

95.011

0.512
  comFA/cflA Streptococcus mitis NCTC 12261

52.607

95.692

0.503
  comFA/cflA Streptococcus mitis SK321

51.659

95.692

0.494
  comFA Lactococcus lactis subsp. cremoris KW2

47.118

90.476

0.426
  comFA Latilactobacillus sakei subsp. sakei 23K

39.604

91.61

0.363

Multiple sequence alignment