Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACA027_RS21735 Genome accession   NZ_CP166730
Coordinates   4916963..4918540 (-) Length   525 a.a.
NCBI ID   WP_370680263.1    Uniprot ID   -
Organism   Comamonas sp. GB3 AK4-5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4911963..4923540
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA027_RS21720 (ACA027_21720) - 4913432..4915270 (+) 1839 WP_370680260.1 methyl-accepting chemotaxis protein -
  ACA027_RS21725 (ACA027_21725) - 4915301..4915654 (+) 354 WP_370680261.1 hypothetical protein -
  ACA027_RS21730 (ACA027_21730) - 4915729..4916736 (-) 1008 WP_370680262.1 ribokinase -
  ACA027_RS21735 (ACA027_21735) rpoN/rpoN1 4916963..4918540 (-) 1578 WP_370680263.1 RNA polymerase factor sigma-54 Machinery gene
  ACA027_RS21740 (ACA027_21740) lptB 4918609..4919382 (-) 774 WP_370680264.1 LPS export ABC transporter ATP-binding protein -
  ACA027_RS21745 (ACA027_21745) lptA 4919379..4920026 (-) 648 WP_370680265.1 lipopolysaccharide transport periplasmic protein LptA -
  ACA027_RS21750 (ACA027_21750) - 4920309..4920962 (-) 654 WP_370680266.1 thiol:disulfide interchange protein DsbA/DsbL -
  ACA027_RS21755 (ACA027_21755) - 4921176..4921853 (-) 678 WP_370680267.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 525 a.a.        Molecular weight: 57848.08 Da        Isoelectric Point: 5.2067

>NTDB_id=1035062 ACA027_RS21735 WP_370680263.1 4916963..4918540(-) (rpoN/rpoN1) [Comamonas sp. GB3 AK4-5]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDDNPFLELSPEEAPREDFGLSQSDAPAQADDYGAEQAAL
SGASDAGAESAEGPNNAESAADFDAPDWDGDGGHDLQPDDSEWGGDAPARGALKDGEEGISALDLARAHESLTDHLLRQA
LSLHLSELDGHVLRYLIENLNDDGYLEDTLQSLAEGLAGCEDPERLEELVHRFTVALRLLHSLEPVGVGARDLGECLQLQ
LQHLQQRREADPAVLETALAICAQPLDLLARRDVRRLLQLTGHHEERVRAAMALIARLEPRPGRRFADVERHIVVPDVIV
TRSSRRASEGTPQFNVQLNADVMPRLRVHDIYAGALRGHKGSEGHAGLQQRLQEARWFIKNVQQRFDTILRVSRAIVERQ
KNFFVHGDLAMRPLVLRDIADELGLHESTISRVTTAKYMATPHGTYELKYFFGSGLGTETGGNASSTAVRALIKQFVSAE
SVKKPLSDSQIADMLKEQGIDCARRTVAKYRDALKIAPANLRKKL

Nucleotide


Download         Length: 1578 bp        

>NTDB_id=1035062 ACA027_RS21735 WP_370680263.1 4916963..4918540(-) (rpoN/rpoN1) [Comamonas sp. GB3 AK4-5]
TTGAAGCCAGGTTTGTCCTTGCGAGTCTCGCAACATTTGGCTTTGACGCCGCAATTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCCACCCTGGAGCTGGCCCAGGAAGTCGAGCAGATGCTCGACGACAACCCTTTTCTGGAGCTTTCCCCCGAAGAAG
CCCCGCGCGAAGACTTCGGGCTGTCCCAATCCGATGCCCCGGCCCAGGCCGATGACTACGGGGCCGAACAAGCTGCACTT
TCAGGAGCTTCAGACGCTGGTGCAGAAAGCGCTGAAGGCCCAAACAATGCAGAAAGTGCCGCCGATTTCGATGCCCCCGA
CTGGGACGGCGACGGCGGCCACGACCTGCAGCCCGATGACAGCGAATGGGGCGGGGACGCCCCGGCGCGCGGCGCGCTCA
AGGACGGGGAGGAGGGCATCAGCGCACTGGATCTGGCGCGTGCCCATGAATCGCTGACCGACCACCTGCTGCGCCAGGCG
CTGTCGCTGCACCTGTCCGAGCTGGACGGCCATGTGCTGCGCTATCTGATTGAAAACCTGAACGACGACGGCTATCTGGA
AGACACGCTGCAGTCCCTGGCCGAAGGCTTGGCGGGCTGCGAAGACCCGGAGCGCCTGGAAGAGCTGGTGCACCGCTTCA
CCGTGGCGCTGCGTTTGCTGCACAGCCTGGAACCCGTGGGCGTGGGGGCGCGTGATCTGGGCGAATGCCTGCAACTGCAG
CTGCAGCATTTGCAGCAGCGCCGCGAAGCCGATCCCGCCGTACTGGAAACGGCGCTGGCCATTTGCGCACAGCCGCTGGA
CCTGCTGGCGCGGCGCGATGTGCGCCGTCTGCTGCAGCTCACCGGCCACCATGAGGAGCGCGTTCGCGCCGCCATGGCGC
TGATTGCCCGGCTCGAGCCCCGGCCCGGCCGGCGCTTTGCCGATGTGGAGCGCCACATCGTTGTGCCGGATGTGATCGTC
ACCCGCTCCAGTCGCCGTGCCAGCGAAGGCACACCCCAGTTCAATGTGCAGCTCAATGCCGATGTGATGCCGCGGCTGCG
CGTGCACGACATCTATGCCGGTGCGTTGCGCGGCCACAAGGGCAGCGAAGGCCATGCCGGCCTGCAGCAGCGCCTGCAGG
AGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTTGACACCATCTTGCGTGTCTCGCGCGCCATCGTGGAGCGGCAG
AAGAATTTCTTTGTGCATGGCGACCTGGCCATGCGCCCGCTGGTGTTGCGCGATATTGCCGATGAGCTGGGCCTGCATGA
GTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCATGGCACTTATGAGCTGAAGTACTTTTTCGGCT
CCGGCCTGGGCACGGAAACCGGCGGCAATGCCTCGAGCACGGCAGTGCGGGCGCTGATCAAGCAATTCGTCTCGGCAGAG
AGCGTGAAAAAGCCGCTGTCGGACAGCCAGATCGCCGACATGCTCAAGGAACAGGGCATTGATTGCGCCCGCCGCACCGT
GGCCAAATACCGCGATGCGCTGAAGATTGCACCAGCCAATCTGCGCAAAAAGCTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-352)

Sigma-54, DNA binding domain (Sigma54_DBD)

(368-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.789

100

0.524

Multiple sequence alignment