Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   HUW69_RS01245 Genome accession   NZ_LT992490
Coordinates   279355..281559 (+) Length   734 a.a.
NCBI ID   WP_174566421.1    Uniprot ID   -
Organism   Vibrio cholerae strain 4295STDY6534200     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274355..286559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW69_RS01225 (SAMEA4374365_00254) - 275435..276601 (-) 1167 WP_000213242.1 O-succinylhomoserine (thiol)-lyase -
  HUW69_RS01230 (SAMEA4374365_00255) metJ 276869..277186 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  HUW69_RS01235 (SAMEA4374365_00256) - 277297..278574 (-) 1278 WP_029627698.1 malic enzyme-like NAD(P)-binding protein -
  HUW69_RS01240 (SAMEA4374365_00257) rpmE 278882..279100 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  HUW69_RS01245 (SAMEA4374365_00258) priA 279355..281559 (+) 2205 WP_174566421.1 primosomal protein N' Machinery gene
  HUW69_RS01250 (SAMEA4374365_00259) cytR 281865..282872 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  HUW69_RS01255 (SAMEA4374365_00260) - 283044..283595 (+) 552 WP_001891621.1 SPOR domain-containing protein -
  HUW69_RS01260 (SAMEA4374365_00261) hslV 283732..284289 (+) 558 WP_000208249.1 ATP-dependent protease subunit HslV -
  HUW69_RS01265 (SAMEA4374365_00262) hslU 284355..285686 (+) 1332 WP_001293366.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82126.50 Da        Isoelectric Point: 8.4848

>NTDB_id=1034857 HUW69_RS01245 WP_174566421.1 279355..281559(+) (priA) [Vibrio cholerae strain 4295STDY6534200]
MRPTIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKFVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSSTLKALVEKGWIECEERKPVARPWPQELEAKVDKPKLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALSGKYHHLQLTQRAGNALPTRNHALDVKGLYL
DSGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLHQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPVLQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=1034857 HUW69_RS01245 WP_174566421.1 279355..281559(+) (priA) [Vibrio cholerae strain 4295STDY6534200]
ATGCGACCCACCATTGCCCGAGTTTGCCTGCCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTACAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATTCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGTCAGTTTTATCATTATCCGCTCGGAGAGACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAGGCGGCAGAGTTGACTAGCCATAAGGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAATTGATGCAAGGGGTGA
AGCGTGGTGCAGTAAAGCAAGCGCAAGTCCTTCACCTGCTGCAGCACGGCGCTTTATCGCACCAAACTTTGCTTGATGAA
GAGGTGAGCAGTTCGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATCGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCCAAAGTCGATAAACCTAAGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGTTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACG
CCGGTATTGGCGCGTGGTGAACAAGCCTTGGTGTTAGTGCCGGAAATTGGTTTAACCCCACAAACCATCAACCGTTTTCG
CCAACGTTTTAATGTCCCCGTTGAAGTTATGCACTCGGCGCTCAACGATACTGAACGCCTCAACGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCTGCGCTGCTCACGCCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGCCTGCGTTATCACGCTCGAGATGTGGCTGTGATGCGCGCGCA
TTTGGAAAATATTCCGATCGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCAGTGGTAAATACC
ATCACTTGCAACTGACGCAGCGCGCCGGTAATGCGCTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTA
GACAGTGGCCTTTCTGCGCCTTTGATTGCAGAAATGCGCCGCCATTTGTCGGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCCTTGATGTGTCACGAATGTGGCTGGATTGCGGAGTGTCAGCGCTGCGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCATCATTGTGGTTCACAGCGTCCTGTTCTCCATCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGAATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTAATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTTGCGCTATTAGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGAGCCAGTAAGCCTGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTACTGCATAAGG
ATTACCACCACTTTGCTTTGACGGCGTTGGAAGAGCGCAAACTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCTGAAGCCAATCACACCGCTCAAGTGGAAGATTTTCTGCGCCAAGTACGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTTGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACTCTGATGCAGAAAATTTTGCAAAGCGCACGCCCCGTATTGCAACAGTTACCGTTGGCGAGC
AAAGTACGTTGGTCGATCGATATTGACCCACAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

DEAD/DEAH box helicase (DEAD)

(205-364)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.572

100

0.439

Multiple sequence alignment