Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   AB9V60_RS03295 Genome accession   NZ_CP166624
Coordinates   742177..743769 (-) Length   530 a.a.
NCBI ID   WP_024692602.1    Uniprot ID   A0AAD0IBQ2
Organism   Pseudomonas syringae pv. atrofaciens strain GM 2231     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 737177..748769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9V60_RS03280 (AB9V60_03280) - 739190..739597 (+) 408 WP_017279558.1 type IV pilin protein -
  AB9V60_RS03285 (AB9V60_03285) thiO 739623..740726 (+) 1104 WP_122242554.1 glycine oxidase ThiO -
  AB9V60_RS03290 (AB9V60_03290) pilR 740837..742174 (-) 1338 WP_017279844.1 sigma-54 dependent transcriptional regulator Regulator
  AB9V60_RS03295 (AB9V60_03295) pilS 742177..743769 (-) 1593 WP_024692602.1 PAS domain-containing sensor histidine kinase Regulator
  AB9V60_RS03300 (AB9V60_03300) - 743759..743998 (-) 240 WP_032607804.1 PP0621 family protein -
  AB9V60_RS03305 (AB9V60_03305) - 744118..745140 (-) 1023 WP_003344571.1 outer membrane protein assembly factor BamD -
  AB9V60_RS03310 (AB9V60_03310) rluD 745290..746252 (+) 963 WP_002551999.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  AB9V60_RS03315 (AB9V60_03315) pgeF 746249..746977 (+) 729 WP_017279845.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58016.91 Da        Isoelectric Point: 6.8620

>NTDB_id=1034841 AB9V60_RS03295 WP_024692602.1 742177..743769(-) (pilS) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
MTADSPSLVSPQAQRILRLYHLYRLTIGVVLVLLISSSLETELLQLANPNLFRSGCWLYLVFNILVVVLLERPSRQAQFF
GLAMTDAIMLSALFYAAGGPSSGIGNLLIASVAISNVLLRGRIGLLIAAVSAIGIIYLTFYISFGTPSVSNDYVQAGSLG
ALCFAAALLVQALTARMQVSEVLAEQRAADVDSLEALNALILQRMRTGILVLDANHQVLLANQSALHLLSQEQLVGQMID
TCSPQLVERLRQWLVNPIMVPRSITTAPVGLVLQPSFATLNRGDQRQTLVFLEDLSQIAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAADRRLGQIIHEQSGRIDRVIENVLQLSRRREAQPELLDLKTWLEHFVAEFRSSAAPN
QSIHVDISPGTLTTRMDSGQLTQIMTNLVQNGLRYSGKNHPSAQVWLKLHNEPRSDLPTLEVLDDGPGVSEQHLSKIFEP
FFTTESKGTGLGLYLSRELCESNQAHLEYAPRPTGGSCLRITFAHPFKTS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1034841 AB9V60_RS03295 WP_024692602.1 742177..743769(-) (pilS) [Pseudomonas syringae pv. atrofaciens strain GM 2231]
GTGACCGCTGATTCACCGTCCCTTGTCAGCCCGCAGGCACAGCGGATTCTGCGGCTGTACCACCTTTATCGACTGACCAT
CGGCGTCGTACTGGTGCTGTTGATTTCCAGCAGCCTGGAAACCGAGCTGCTGCAACTGGCCAACCCGAACCTATTCCGCT
CGGGCTGCTGGCTGTATCTGGTGTTCAACATTCTGGTCGTCGTGCTGCTGGAACGCCCCAGCCGTCAGGCACAGTTTTTC
GGCCTGGCGATGACTGACGCGATCATGCTCTCCGCGCTGTTCTATGCAGCGGGCGGGCCTTCAAGCGGTATCGGCAACCT
GTTGATTGCCTCGGTGGCGATCAGCAACGTGTTGCTGCGCGGCCGTATCGGCTTGTTGATCGCTGCGGTTTCGGCCATCG
GCATCATCTACCTGACGTTCTACATCAGCTTCGGCACACCCTCGGTCTCCAACGACTACGTGCAGGCTGGCTCGCTGGGC
GCTTTATGCTTCGCCGCCGCCCTGCTGGTGCAGGCGCTGACAGCGCGCATGCAGGTTAGCGAGGTACTGGCCGAACAGCG
CGCAGCGGATGTCGACAGTCTCGAAGCACTCAACGCACTCATTTTGCAGCGCATGCGTACCGGCATTCTGGTGCTGGATG
CCAATCACCAGGTGCTGCTGGCCAACCAGAGTGCCTTGCACCTGCTGAGCCAGGAGCAACTGGTCGGCCAGATGATCGAC
ACCTGCTCGCCGCAGCTGGTTGAACGCCTGCGCCAGTGGCTCGTCAATCCGATCATGGTGCCGCGCAGTATCACCACCGC
CCCCGTCGGGCTGGTGCTGCAACCGAGCTTTGCCACACTCAACCGTGGCGATCAGCGTCAGACGCTGGTTTTTCTCGAAG
ACCTGTCGCAGATCGCCCACCAGGCGCAGCAGCTCAAGCTCGCCGCACTGGGACGCCTGACCGCCGGGATTGCCCATGAG
ATACGCAACCCGCTGGGCGCCGTCAGTCATGCGGCTCAACTGCTTAATGAATCGGAAGAGCTCAGTGCAGCGGATCGACG
CCTGGGCCAGATCATTCATGAGCAGTCGGGACGCATTGACCGAGTCATCGAAAATGTCCTGCAATTGTCGCGCAGACGCG
AAGCGCAACCCGAACTGCTCGATCTGAAAACCTGGCTGGAGCACTTTGTCGCTGAATTTCGCAGTTCCGCAGCGCCCAAT
CAGTCTATTCATGTCGACATCAGCCCCGGCACGCTGACAACCCGCATGGATTCTGGACAGCTGACGCAAATCATGACCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGGCAAGAATCATCCGTCAGCGCAGGTCTGGCTGAAACTGCACAATGAACCGA
GAAGCGATTTGCCTACGCTGGAAGTGCTGGACGATGGGCCGGGGGTCAGCGAGCAACACCTGAGCAAGATCTTCGAACCG
TTTTTCACCACAGAAAGCAAAGGCACTGGCCTTGGTCTATATCTGTCACGTGAACTGTGCGAAAGCAATCAGGCGCATCT
GGAATATGCGCCCCGCCCCACGGGTGGCAGCTGCCTGCGCATTACCTTCGCCCACCCGTTCAAGACGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

61.017

100

0.611

Multiple sequence alignment