Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DXE38_RS00395 Genome accession   NZ_LT992477
Coordinates   66240..66737 (+) Length   165 a.a.
NCBI ID   WP_001825340.1    Uniprot ID   -
Organism   Staphylococcus aureus isolate 22_LA_562     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 61240..71737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DXE38_RS00355 - 61284..62270 (+) 987 WP_000161315.1 ROK family glucokinase -
  DXE38_RS00360 - 62270..62599 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DXE38_RS00365 - 62596..63219 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DXE38_RS00370 comGA 63271..64245 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DXE38_RS00375 comGB 64217..65287 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  DXE38_RS00380 comGC 65301..65612 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DXE38_RS00385 comGD 65590..66036 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  DXE38_RS00390 comGE 66023..66322 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  DXE38_RS00395 comGF 66240..66737 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  DXE38_RS00400 - 66928..68100 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  DXE38_RS00405 - 68167..68313 (+) 147 WP_001789879.1 hypothetical protein -
  DXE38_RS00410 - 68303..68827 (+) 525 WP_001015120.1 shikimate kinase -
  DXE38_RS00415 gcvT 68986..70077 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DXE38_RS00420 gcvPA 70097..71443 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19179.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1034597 DXE38_RS00395 WP_001825340.1 66240..66737(+) (comGF) [Staphylococcus aureus isolate 22_LA_562]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1034597 DXE38_RS00395 WP_001825340.1 66240..66737(+) (comGF) [Staphylococcus aureus isolate 22_LA_562]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97