Detailed information NTG1033905

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB9D63_RS24735
• Genome accession: NZ_CP166234
• Coordinates: 5147683..5148480 (+)
• Length: 265 a.a.
• NCBI ID: WP_032694399.1
• Uniprot ID: A0A2J5PD60
• Organism: Klebsiella michiganensis strain 2022CK-00623
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5142683..5153480

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9D63_RS24700 (AB9D63_24705)	gspH	5142824..5143336 (+)	513	WP_038424080.1	type II secretion system minor pseudopilin GspH	-
AB9D63_RS24705 (AB9D63_24710)	gspI	5143333..5143698 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
AB9D63_RS24710 (AB9D63_24715)	gspJ	5143695..5144291 (+)	597	WP_014837428.1	type II secretion system minor pseudopilin GspJ	-
AB9D63_RS24715 (AB9D63_24720)	gspK	5144288..5145268 (+)	981	WP_032720565.1	type II secretion system minor pseudopilin GspK	-
AB9D63_RS24720 (AB9D63_24725)	gspL	5145261..5146454 (+)	1194	WP_032720564.1	type II secretion system protein GspL	-
AB9D63_RS24725 (AB9D63_24730)	gspM	5146447..5146932 (+)	486	WP_038424078.1	type II secretion system protein GspM	-
AB9D63_RS24730 (AB9D63_24735)	-	5146922..5147680 (+)	759	WP_038424077.1	type II secretion system protein N	-
AB9D63_RS24735 (AB9D63_24740)	pilD	5147683..5148480 (+)	798	WP_032694399.1	A24 family peptidase	Machinery gene
AB9D63_RS24740 (AB9D63_24745)	hrpB	5148578..5151007 (-)	2430	WP_038424076.1	ATP-dependent helicase HrpB	-
AB9D63_RS24745 (AB9D63_24750)	thpR	5151080..5151616 (+)	537	WP_014227968.1	RNA 2',3'-cyclic phosphodiesterase	-
AB9D63_RS24750 (AB9D63_24755)	sfsA	5151616..5152332 (+)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
AB9D63_RS24755 (AB9D63_24760)	dksA	5152497..5152952 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29150.77 Da
• Isoelectric Point: 8.3410

>NTDB_id=1033905 AB9D63_RS24735 WP_032694399.1 5147683..5148480(+) (pilD) [Klebsiella michiganensis strain 2022CK-00623]
MENIALLPEFAAQFPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=1033905 AB9D63_RS24735 WP_032694399.1 5147683..5148480(+) (pilD) [Klebsiella michiganensis strain 2022CK-00623]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATTCCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTAAATCTCTGCC
TCCCCGCCTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGTCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A2J5PD60

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.808	98.491	0.491
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434