Detailed information NTG1033743

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB9B78_RS22385
• Genome accession: NZ_CP166210
• Coordinates: 4675213..4676010 (+)
• Length: 265 a.a.
• NCBI ID: WP_086530433.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain 2021CK-01841
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4670213..4681010

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB9B78_RS22350 (AB9B78_22370)	gspH	4670354..4670866 (+)	513	WP_004098708.1	type II secretion system minor pseudopilin GspH	-
AB9B78_RS22355 (AB9B78_22375)	gspI	4670863..4671228 (+)	366	WP_004098707.1	type II secretion system minor pseudopilin GspI	-
AB9B78_RS22360 (AB9B78_22380)	gspJ	4671225..4671821 (+)	597	WP_047724421.1	type II secretion system minor pseudopilin GspJ	-
AB9B78_RS22365 (AB9B78_22385)	gspK	4671818..4672798 (+)	981	WP_086530436.1	type II secretion system minor pseudopilin GspK	-
AB9B78_RS22370 (AB9B78_22390)	gspL	4672791..4673984 (+)	1194	WP_109212802.1	type II secretion system protein GspL	-
AB9B78_RS22375 (AB9B78_22395)	gspM	4673977..4674462 (+)	486	WP_009654623.1	type II secretion system protein GspM	-
AB9B78_RS22380 (AB9B78_22400)	-	4674452..4675210 (+)	759	WP_197663591.1	type II secretion system protein N	-
AB9B78_RS22385 (AB9B78_22405)	pilD	4675213..4676010 (+)	798	WP_086530433.1	A24 family peptidase	Machinery gene
AB9B78_RS22390 (AB9B78_22410)	hrpB	4676108..4678537 (-)	2430	WP_049073913.1	ATP-dependent helicase HrpB	-
AB9B78_RS22395 (AB9B78_22415)	thpR	4678610..4679146 (+)	537	WP_049073914.1	RNA 2',3'-cyclic phosphodiesterase	-
AB9B78_RS22400 (AB9B78_22420)	sfsA	4679146..4679862 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
AB9B78_RS22405 (AB9B78_22425)	dksA	4680027..4680482 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29064.69 Da
• Isoelectric Point: 7.5684

>NTDB_id=1033743 AB9B78_RS22385 WP_086530433.1 4675213..4676010(+) (pilD) [Klebsiella michiganensis strain 2021CK-01841]
METIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLFL
KGRSRCCGEPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=1033743 AB9B78_RS22385 WP_086530433.1 4675213..4676010(+) (pilD) [Klebsiella michiganensis strain 2021CK-01841]
ATGGAAACTATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGTTTCCTGTTTCTT
AAGGGACGTTCGCGCTGCTGCGGGGAACCCATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTTTAT
GCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGGCTAATTTTCGTATCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGTTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCTGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	47.431	95.472	0.453
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438