Detailed information NTG1033431

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: AB8806_RS00220
• Genome accession: NZ_CP166102
• Coordinates: 45455..46582 (+)
• Length: 375 a.a.
• NCBI ID: WP_013213631.1
• Uniprot ID: A0A1U9VHA8
• Organism: Ralstonia syzygii subsp. celebesensis strain NCPPB 3728
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 40455..51582

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB8806_RS00195 (AB8806_00195)	-	40830..41078 (-)	249	WP_078222383.1	YfhL family 4Fe-4S dicluster ferredoxin	-
AB8806_RS00200 (AB8806_00200)	coaD	41129..41635 (-)	507	WP_003262550.1	pantetheine-phosphate adenylyltransferase	-
AB8806_RS00205 (AB8806_00205)	rsmD	41831..42478 (-)	648	WP_075465409.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
AB8806_RS00210 (AB8806_00210)	-	42558..43901 (-)	1344	WP_013213629.1	pitrilysin family protein	-
AB8806_RS00215 (AB8806_00215)	-	43901..45394 (-)	1494	WP_078222382.1	pitrilysin family protein	-
AB8806_RS00220 (AB8806_00220)	pilA	45455..46582 (+)	1128	WP_013213631.1	signal recognition particle-docking protein FtsY	Machinery gene
AB8806_RS00225 (AB8806_00225)	-	46579..47217 (-)	639	WP_078222381.1	LysE/ArgO family amino acid transporter	-
AB8806_RS00230 (AB8806_00230)	maiA	47214..47864 (-)	651	WP_078222380.1	maleylacetoacetate isomerase	-
AB8806_RS00235 (AB8806_00235)	-	47868..48569 (-)	702	WP_078222379.1	fumarylacetoacetate hydrolase family protein	-
AB8806_RS00240 (AB8806_00240)	-	48720..49346 (+)	627	WP_013213635.1	ANTAR domain-containing response regulator	-
AB8806_RS00245 (AB8806_00245)	-	49623..50885 (+)	1263	WP_078222378.1	CmpA/NrtA family ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 39819.04 Da
• Isoelectric Point: 6.1635

>NTDB_id=1033431 AB8806_RS00220 WP_013213631.1 45455..46582(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3728]
MFSFWKKRKAEPQPAAEPVPPAAAPEAVQAPAPAPVASPAPAPAPVAVPDTPATPAALDMQADDIETVPTPPVVEQARKG
WMSRLRSGLSKTSKGLTTLFVGVKVDEALFEELETALLMADAGVDATEYLLGELRRRVKAQRIETAEGVKTALRDLLIEL
LHPLEKTMVLGREQPTVIMIAGVNGAGKTTSIGKLCKHFQTYGQSVLLAAGDTFRAAAREQLVIWGQRNNVTVVAQESGD
PAAVIFDAVNAARARGIDIVMADTAGRLPTQLHLMEELKKVRRVIGKAMATAPHETLLVIDANTGQNALAQVKAFDDTLG
LTGLIITKLDGTAKGGILAAIARQRPVPVYFIGVGEQVEDLQPFSAREFADALLG

Nucleotide

• Length: 1128 bp

>NTDB_id=1033431 AB8806_RS00220 WP_013213631.1 45455..46582(+) (pilA) [Ralstonia syzygii subsp. celebesensis strain NCPPB 3728]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCAGAGCCCCAGCCGGCTGCCGAACCGGTGCCGCCCGCCGCCGCACCTGAAGC
GGTACAGGCTCCAGCCCCGGCCCCGGTTGCCTCCCCTGCACCTGCACCCGCCCCGGTTGCCGTGCCGGATACACCGGCCA
CGCCGGCTGCGCTGGACATGCAGGCCGACGATATCGAGACCGTGCCGACGCCGCCCGTGGTGGAGCAGGCGCGCAAGGGC
TGGATGTCGCGCCTGCGCTCGGGCCTGTCGAAGACCAGCAAGGGCCTGACCACGCTGTTCGTCGGCGTGAAGGTGGACGA
GGCGCTGTTCGAGGAGCTGGAAACCGCGCTGCTGATGGCCGATGCAGGCGTCGATGCCACCGAATACCTGCTGGGCGAAC
TGCGCCGCCGTGTGAAAGCCCAGCGCATCGAGACCGCCGAGGGCGTGAAGACCGCGCTGCGCGACCTGCTCATCGAGCTG
CTGCATCCGCTGGAAAAGACCATGGTGCTCGGCCGCGAGCAGCCGACGGTGATCATGATCGCGGGCGTCAACGGCGCCGG
CAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGACCTACGGGCAATCCGTGCTGCTGGCAGCGGGCGACACGT
TCCGTGCTGCCGCGCGCGAGCAGTTGGTGATCTGGGGCCAGCGCAACAACGTGACCGTGGTGGCGCAGGAATCGGGCGAC
CCGGCCGCCGTGATCTTCGACGCGGTGAACGCGGCGCGCGCCCGCGGCATCGATATCGTCATGGCCGACACCGCCGGCCG
CCTGCCGACCCAGCTGCACCTGATGGAAGAACTGAAGAAGGTGCGCCGCGTGATCGGCAAGGCGATGGCGACGGCGCCGC
ACGAGACGTTGCTGGTGATCGACGCCAACACCGGCCAGAATGCGCTGGCGCAGGTGAAAGCCTTCGACGATACGCTCGGC
CTGACCGGCCTGATCATCACCAAGCTGGACGGCACCGCCAAGGGCGGCATCCTGGCCGCCATCGCGCGGCAGCGGCCTGT
ACCGGTGTATTTCATCGGCGTGGGCGAACAGGTGGAAGACCTGCAGCCGTTCTCCGCACGCGAATTCGCCGACGCGCTAC
TGGGCTAG

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (175-375)

• SRP54-type protein, helical bundle domain (SRP54_N) (94-155)

3D structure

• AlphaFold DB: A0A1U9VHA8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	62.951	81.333	0.512