Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AB8E30_RS18305 Genome accession   NZ_CP165965
Coordinates   3458128..3461640 (-) Length   1170 a.a.
NCBI ID   WP_369900703.1    Uniprot ID   -
Organism   Bacillus manliponensis strain jeju3-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3453128..3466640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E30_RS18295 (AB8E30_18305) - 3453374..3454342 (-) 969 WP_369900701.1 sporulation protein -
  AB8E30_RS18300 (AB8E30_18310) addA 3454418..3458131 (-) 3714 WP_369900702.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB8E30_RS18305 (AB8E30_18315) addB 3458128..3461640 (-) 3513 WP_369900703.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB8E30_RS18310 (AB8E30_18320) lepB 3461739..3462305 (-) 567 WP_369900704.1 signal peptidase I -
  AB8E30_RS18315 (AB8E30_18325) - 3462351..3462950 (-) 600 WP_369900705.1 TVP38/TMEM64 family protein -
  AB8E30_RS18320 (AB8E30_18330) cspD 3463161..3463364 (-) 204 WP_034637437.1 cold-shock protein CspD -
  AB8E30_RS18325 (AB8E30_18335) - 3464015..3464590 (-) 576 WP_369900706.1 competence protein ComK -
  AB8E30_RS18330 (AB8E30_18340) - 3464973..3465296 (-) 324 WP_369900707.1 hypothetical protein -
  AB8E30_RS18335 (AB8E30_18345) - 3465786..3465968 (+) 183 WP_369900708.1 hypothetical protein -

Sequence

Protein


Download         Length: 1170 a.a.        Molecular weight: 134477.63 Da        Isoelectric Point: 5.5223

>NTDB_id=1032654 AB8E30_RS18305 WP_369900703.1 3458128..3461640(-) (addB) [Bacillus manliponensis strain jeju3-1]
MSLQFVIGRAGSGKSGLLLHEVQEELKRRPRGNAILYIVPEQMTFQTQQALIRSEEVQSSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVEARKEELHVFQKAAEQNGFFEQLGSMITEFKRYNLTPSNVYTMWQELEANSSGNEQKLLA
NKMYDLQLLYDDFERALIGKYLDSEDYLQLLFEKIPHSEYVKDADIYIDGFHSLSPQELEIVRQLMRYGAKVKITLTLDE
QTLAMPVSELDLFHQTIVTYENVKQIAREEKMVLQPTIFMKEQPRFHSSGLAHLEAYYEARFPKEFEGESNITLYNAVNL
RAEVEGVAREIRRLIAEEGYRYRDIAVLLRNGESYYDLMKTLFVDYNIPHFIDQKRSMSHHPLIECIRSALEVISGNWRY
DAVFRCVKTELLYPLDVNKESMREEMDEFENYCLAYGVQGKRWTSEDPWTYRRYRSLDGGEGAQTNSEREMEEKVNRLRG
VVRTPIIRLQKRLKRATTSMGMCEAVYLFLEELDVPKKLEELRMKAEAEGDFLFATDHDQVWEQVMSLLDTFVEMLGDEK
MSLSMFMNVMTTGLESLQFANIPPSLDQVIIANIDRSRLSNIRAAFIVGANEGVIPAAPTEEGVLSDEEREALVAAGTLL
APTTRRKLLDEQFVIYQTVTCASDKLYLSCPLADEEGKTLLVSSFMKRMKRMFPSIKEQFLTNDVNDISIREQISYVATP
EVTMSYLTQQLQNWKRYGFEGNLSFWWDVYNFYVTSDEWKQKSSRILSSLFYRNRAEKLNNDVSRQLYGEKIKGSVSRME
LFNRCSYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLTVKECEYLSQVVIEELAPLLQRQIL
LSSNRHHYLKHKLQQIIFRTSLVLREHAKASGFVPVDLEVPFGMGGADSLPPMQFELANGVKMEVVGRIDRVDKAEDESG
TYLRIIDYKSSAKALDLTEVYYGLALQMLTYLDVVTSNASKWMKGSGQVSPAGVLYFHIHNPIVEMKGDAGEAEIEQEIL
KKFKMKGLVLGDADVVRLMDHSLTTGSSNIISAGLKKDGSFSARSSIASEQEFDVLQKYVHQKFENIGTHITDGVIDIAP
YKMGNKTACTFCNFRSVCQFDESLEDNEYRSLKDMKDSEALEKMREEAHK

Nucleotide


Download         Length: 3513 bp        

>NTDB_id=1032654 AB8E30_RS18305 WP_369900703.1 3458128..3461640(-) (addB) [Bacillus manliponensis strain jeju3-1]
ATGTCGCTTCAATTTGTAATAGGACGAGCAGGTAGTGGAAAGAGTGGACTTCTTTTACATGAAGTGCAAGAAGAGTTAAA
GCGAAGACCAAGAGGAAATGCGATTTTATATATTGTTCCGGAGCAAATGACGTTCCAAACGCAGCAAGCTTTAATTCGCA
GTGAGGAAGTGCAAAGCTCAATTCGAGCACAAGTATTTAGTTTCTCTCGTTTAGCATGGAAAGTATTGCAAGAAGTTGGC
GGCGCAAGTCGTCTTCATATTGATGAAGCAGGCGTTCATATGTTGCTTAGAAAAATTGTAGAGGCGCGTAAAGAAGAATT
ACATGTGTTCCAAAAAGCTGCAGAGCAAAATGGATTTTTCGAACAACTTGGCAGCATGATTACGGAATTTAAACGCTACA
ATTTAACACCATCTAACGTATATACGATGTGGCAAGAGTTAGAGGCGAATAGTAGCGGCAATGAACAAAAGTTATTAGCA
AATAAAATGTACGATTTACAGCTTTTATATGATGACTTTGAACGTGCATTAATTGGAAAGTATTTGGATTCAGAAGATTA
TCTACAACTATTATTTGAAAAAATTCCGCACTCAGAGTACGTAAAGGATGCGGACATTTATATAGATGGTTTCCATTCGC
TTTCACCGCAAGAATTAGAAATTGTTCGGCAGCTTATGCGTTATGGTGCAAAAGTGAAAATAACGTTAACGTTAGACGAA
CAGACGCTAGCAATGCCAGTAAGTGAACTGGATTTATTTCATCAAACAATTGTTACGTATGAAAATGTAAAACAAATAGC
TCGTGAAGAGAAAATGGTGTTACAGCCAACGATTTTTATGAAAGAACAACCAAGATTCCATTCATCTGGACTTGCGCATT
TAGAAGCGTATTATGAAGCGCGCTTTCCGAAAGAATTTGAAGGTGAATCAAATATTACGTTATATAACGCTGTGAACTTG
CGTGCTGAAGTAGAGGGAGTGGCCCGTGAAATTCGTCGTCTTATTGCAGAAGAGGGTTATCGTTACCGCGATATTGCAGT
TCTTCTTCGTAATGGAGAGAGTTATTATGACTTAATGAAAACGTTGTTTGTTGATTATAATATTCCACACTTTATCGACC
AAAAGCGTTCGATGTCACATCATCCACTTATTGAATGTATTCGCTCAGCACTTGAAGTTATTAGTGGGAATTGGCGTTAT
GATGCGGTATTTCGCTGCGTGAAAACGGAGCTATTATATCCGTTAGATGTGAATAAAGAAAGCATGCGCGAAGAGATGGA
TGAGTTTGAAAACTATTGTTTAGCGTACGGTGTGCAAGGAAAAAGGTGGACTTCAGAAGACCCATGGACGTACCGTCGTT
ATCGCTCATTAGATGGGGGCGAAGGAGCACAAACGAACAGTGAGCGTGAAATGGAAGAGAAAGTGAACCGCTTACGAGGC
GTCGTGAGAACGCCGATTATTCGTTTGCAAAAGCGTTTAAAGCGTGCTACGACATCAATGGGCATGTGCGAAGCCGTATA
TTTATTTTTAGAAGAATTAGATGTACCGAAGAAGCTTGAAGAGTTACGTATGAAAGCAGAGGCGGAAGGGGATTTCTTGT
TTGCAACAGATCATGATCAAGTGTGGGAGCAAGTAATGAGCCTTCTTGATACGTTCGTTGAGATGCTTGGTGACGAGAAG
ATGTCCTTATCAATGTTCATGAATGTGATGACGACAGGGCTTGAGTCGCTTCAATTCGCAAATATCCCGCCATCATTAGA
TCAGGTTATTATTGCAAATATTGACCGCTCTCGTCTTTCGAACATTCGCGCCGCTTTCATTGTTGGAGCAAATGAAGGAG
TTATTCCGGCGGCACCAACTGAAGAAGGCGTGCTATCAGACGAAGAGAGAGAAGCACTCGTTGCAGCTGGCACCTTACTT
GCCCCAACAACACGTCGCAAACTATTAGATGAACAATTTGTTATTTATCAGACCGTGACGTGTGCATCGGATAAGTTATA
TCTATCTTGCCCACTTGCAGATGAAGAGGGAAAAACACTTCTTGTTTCTAGCTTTATGAAAAGAATGAAAAGAATGTTTC
CTTCTATAAAAGAACAGTTTTTAACAAACGATGTGAATGATATATCGATTCGTGAGCAAATTTCGTACGTTGCAACGCCA
GAAGTTACGATGTCGTATTTGACGCAGCAATTGCAAAATTGGAAACGTTACGGATTTGAAGGGAATTTAAGTTTTTGGTG
GGACGTTTATAATTTCTATGTCACTTCAGACGAATGGAAACAAAAGAGCAGCCGCATTTTATCAAGTTTATTTTACCGCA
ACCGCGCTGAGAAGCTGAACAATGATGTAAGCCGCCAGTTATATGGTGAAAAGATTAAAGGAAGCGTTTCACGTATGGAA
CTCTTTAATCGTTGTTCATATGCGCACTTTGCCCAGCATGGATTGTCGTTACGTGAGCGTGATATTTTCAAATTAGATGC
GCCTGATATCGGGGAACTTTTCCACGCTGCTTTAAAGAAAATTGCTGATAAATTGTTACGAGAAAATCGTACGTGGGCAG
ATTTAACAGTAAAAGAATGCGAGTATTTATCACAAGTTGTTATTGAAGAATTAGCGCCGTTATTGCAGCGCCAAATTTTA
TTAAGTTCGAATCGTCATCACTATTTAAAGCATAAATTGCAGCAAATTATTTTCCGTACATCACTTGTACTTCGTGAACA
TGCAAAGGCAAGTGGGTTCGTTCCAGTTGACCTAGAAGTACCATTTGGTATGGGCGGAGCAGACTCCTTACCACCGATGC
AATTTGAACTGGCAAACGGTGTGAAAATGGAAGTGGTCGGCCGCATTGACCGCGTGGATAAAGCGGAAGATGAGAGCGGC
ACGTACTTACGTATTATCGATTATAAATCAAGTGCAAAAGCGTTAGATTTAACAGAAGTGTATTACGGTTTAGCACTGCA
AATGTTGACGTACTTAGATGTTGTGACATCAAATGCGAGCAAATGGATGAAAGGATCAGGACAAGTATCGCCTGCTGGTG
TTCTATATTTCCATATTCATAATCCAATTGTGGAAATGAAAGGCGACGCAGGCGAAGCTGAAATTGAACAAGAGATTTTA
AAGAAATTTAAAATGAAAGGTCTTGTTCTTGGTGATGCAGATGTTGTTCGTTTAATGGATCACTCATTAACGACAGGAAG
CTCAAATATTATTTCTGCTGGCTTAAAGAAAGATGGGAGCTTTAGTGCAAGGTCAAGTATTGCAAGTGAACAAGAATTTG
ATGTACTACAAAAGTACGTTCATCAAAAGTTTGAGAATATCGGAACGCACATTACAGACGGTGTCATCGATATTGCACCG
TATAAGATGGGGAATAAAACAGCGTGTACATTCTGTAACTTCCGCTCTGTTTGTCAATTTGATGAGTCGTTAGAAGATAA
TGAATATAGATCATTAAAAGATATGAAAGATAGTGAAGCACTTGAAAAGATGAGAGAGGAGGCCCATAAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

50.769

100

0.508

Multiple sequence alignment