Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   AB8E30_RS18300 Genome accession   NZ_CP165965
Coordinates   3454418..3458131 (-) Length   1237 a.a.
NCBI ID   WP_369900702.1    Uniprot ID   -
Organism   Bacillus manliponensis strain jeju3-1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3449418..3463131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E30_RS18255 (AB8E30_18265) - 3449691..3450590 (-) 900 WP_369900695.1 fumarylacetoacetate hydrolase family protein -
  AB8E30_RS18260 (AB8E30_18270) - 3450832..3451008 (+) 177 WP_369900696.1 aspartyl-phosphate phosphatase Spo0E family protein -
  AB8E30_RS18265 (AB8E30_18275) - 3451212..3451433 (+) 222 WP_369900697.1 spore germination protein -
  AB8E30_RS18270 (AB8E30_18280) - 3451450..3451653 (+) 204 WP_369900698.1 spore germination protein GerPB -
  AB8E30_RS18275 (AB8E30_18285) - 3451715..3452329 (+) 615 WP_034637459.1 spore germination protein GerPC -
  AB8E30_RS18280 (AB8E30_18290) - 3452334..3452528 (+) 195 WP_369900699.1 spore gernimation protein GerPD -
  AB8E30_RS18285 (AB8E30_18295) - 3452540..3452926 (+) 387 WP_369900700.1 spore germination protein GerPE -
  AB8E30_RS18290 (AB8E30_18300) - 3452972..3453187 (+) 216 WP_034637449.1 spore germination protein -
  AB8E30_RS18295 (AB8E30_18305) - 3453374..3454342 (-) 969 WP_369900701.1 sporulation protein -
  AB8E30_RS18300 (AB8E30_18310) addA 3454418..3458131 (-) 3714 WP_369900702.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB8E30_RS18305 (AB8E30_18315) addB 3458128..3461640 (-) 3513 WP_369900703.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB8E30_RS18310 (AB8E30_18320) lepB 3461739..3462305 (-) 567 WP_369900704.1 signal peptidase I -
  AB8E30_RS18315 (AB8E30_18325) - 3462351..3462950 (-) 600 WP_369900705.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1237 a.a.        Molecular weight: 141872.13 Da        Isoelectric Point: 4.9548

>NTDB_id=1032653 AB8E30_RS18300 WP_369900702.1 3454418..3458131(-) (addA) [Bacillus manliponensis strain jeju3-1]
MINNLPAKPEGSQWTDDQWKAIVADGRDILVAAAAGSGKTAVLVERIIRKIIREENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLADNPASQHIRKQLSLLNRASISTIHSFCLQVIRGYYYMLDIDPRFRIANETENELLKEEVLGDILEEEYSQE
NNTIFFELVDRYTSDRNDDDLQRMILALHTESRAHPDPNRWLDKLVDAYDVTGKSIEDLMYATYLLDDAKIQLQTAKQYV
EKAIELAMLPDGPAPRVETLQADLLLLEGLIQASYQSWQSTYEVMQNVSWQTLKRIKKADYNEDVVAQVDTLRAKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLGKLVSLVKEFAERFQVMKQGKGMVDFTDLEHFCLQILSERSRDGELHPSPVALQ
YRNKFAEVLVDEYQDVNFVQESIVKLVTKESEEEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTPEGVDGGMKIDLAK
NFRSRQEVLAGTNYVFKQIMSESVGEINYDEDAELKLGASYPEGLDTAGELILISEPDNEGADDGDLGLEKAQLEARLIA
QRIKEVVDSSYEVYDRKTGMRPVTYRDFVILLRSMPWAPQIMEELKQQGIPVYAELATGYFEATEVNIMMNVFRVIDNPM
QDIPLAAVLRSPIVGLTDEELATLRTHGKKGSFYEVLRSFLTEASLDNERGLYEKVSLFYEQLKGWREFARQQSLSDLIW
KVYRETGYYDFVGGLPAGKQRQANLRVLYDRARQFEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTIHK
SKGLEFPVVFVAGLGRKFNTQDLMKRFLLHKDLGFGSQFIDPQKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTRAK
EKLILIGTVKGKGKEMEKWLEARDHAQWLLPDYVRAGATCYLDWIAPSLFRHGDSESLLELGQGLIPDDIYRYGASFKVE
AIDGSTLQAPVLLKEEKRDLLEALRAKEPVSLESEQKEDVYDKLTWQYEFRDATVHRAKQSVTEIKRNYQVQEGSDGSFI
KKLRAPIQARPRFMEKKGLTYAERGTAVHAVMQYVDLKKPITVESVAEQIVEMVNKELLTYEQAEEIQAEKIVSFFETEL
GRRLVAAKSVEREVPFTMMLPAEEAYQDWSGSPGESVLVQGVIDCMVEEEDGIVLIDFKTDTIEGRFPGGFEQARPVLEE
RYHVQLSLYRKALEKSLHQPVRETYLYFFDGSHVLKI

Nucleotide


Download         Length: 3714 bp        

>NTDB_id=1032653 AB8E30_RS18300 WP_369900702.1 3454418..3458131(-) (addA) [Bacillus manliponensis strain jeju3-1]
ATGATAAATAACTTACCAGCGAAGCCAGAAGGTAGTCAATGGACAGATGATCAGTGGAAAGCGATTGTTGCTGACGGGCG
TGATATTTTAGTGGCGGCAGCAGCTGGATCAGGGAAAACAGCGGTTCTTGTTGAACGTATTATTCGCAAAATTATTCGCG
AGGAAAATCCAGTTGATGTAGACCGTTTGCTCGTTGTTACATTTACGAATGCAGCAGCGCAAGAGATGAAAAATAGAATT
GGGGAAGCGTTAGAGAAAGTGTTAGCAGATAATCCAGCTTCGCAGCATATTCGAAAACAACTAAGTTTATTAAACCGAGC
TTCCATTTCGACAATTCACTCGTTTTGTTTGCAAGTAATTCGTGGTTATTACTATATGCTTGATATTGATCCACGTTTTC
GCATTGCAAACGAAACAGAGAATGAGCTGTTAAAAGAAGAAGTGCTTGGAGACATATTAGAAGAAGAGTATAGTCAAGAA
AATAATACCATTTTCTTTGAACTTGTTGATCGTTATACGAGCGACCGTAATGACGATGACTTGCAGCGTATGATTTTAGC
GCTGCATACAGAATCAAGAGCGCATCCAGACCCAAATCGCTGGCTTGATAAATTAGTAGATGCATACGATGTGACAGGAA
AATCGATTGAAGACTTAATGTATGCGACGTATTTACTAGATGATGCAAAGATTCAATTACAAACGGCGAAGCAATATGTT
GAAAAAGCAATTGAACTTGCGATGTTACCAGACGGACCAGCGCCGCGTGTTGAAACATTGCAGGCGGATCTTCTCTTACT
TGAAGGGCTAATTCAGGCGTCTTATCAGTCGTGGCAAAGCACATATGAAGTGATGCAAAACGTTTCTTGGCAAACGTTAA
AGCGTATAAAGAAAGCAGACTATAACGAAGATGTGGTCGCTCAAGTTGATACGCTGCGCGCTAAAGCGAAAGATGAAGTG
AAAAAGTTACAAGAGGAGTTATTTAGCCGTAAACCAGAAAGTTTCTTACGTGATTTTCAAGATATGCATCCTGTGCTAGG
GAAGCTTGTTTCACTTGTGAAAGAATTTGCAGAACGTTTCCAAGTGATGAAACAAGGAAAAGGGATGGTTGATTTTACAG
ATTTAGAGCATTTTTGTCTGCAAATTTTAAGTGAGCGCTCTCGCGATGGTGAGCTTCATCCATCACCAGTTGCACTGCAA
TACCGCAATAAATTTGCTGAAGTGCTCGTCGATGAGTATCAGGACGTAAACTTCGTGCAAGAATCGATTGTGAAGCTTGT
GACGAAAGAGAGTGAAGAAGAAGGAAATCTGTTCATGGTTGGCGATGTAAAGCAGTCGATCTACCGTTTCCGTTTAGCAG
AACCAGGGCTGTTTTTAGGGAAATATAAACGCTTTACCCCTGAAGGCGTAGATGGCGGAATGAAAATTGATTTAGCGAAA
AACTTCCGCAGTCGTCAAGAAGTACTAGCTGGTACAAACTACGTTTTCAAACAAATTATGAGTGAATCTGTCGGGGAAAT
TAATTACGACGAAGATGCGGAATTAAAGCTTGGTGCAAGTTATCCAGAAGGCTTAGATACAGCAGGGGAACTTATTTTAA
TTAGCGAGCCTGATAACGAAGGAGCAGATGATGGAGATTTAGGATTGGAAAAAGCGCAGCTAGAAGCACGTCTTATTGCG
CAGCGAATTAAAGAGGTGGTCGATTCTAGCTACGAAGTATACGACCGAAAAACAGGTATGCGTCCTGTAACGTATCGTGA
TTTTGTTATTTTACTGCGTTCGATGCCATGGGCACCGCAAATTATGGAAGAGCTAAAGCAACAAGGTATTCCTGTATACG
CAGAGCTTGCGACAGGTTACTTTGAAGCGACAGAAGTAAATATCATGATGAACGTCTTTCGTGTTATCGATAATCCGATG
CAAGATATTCCGCTTGCGGCAGTGCTTCGATCTCCAATTGTTGGGCTGACAGATGAAGAACTTGCGACGCTTCGTACGCA
CGGAAAGAAAGGTTCATTTTATGAAGTGTTACGCTCTTTCTTAACAGAAGCGTCACTAGATAATGAAAGAGGACTATATG
AAAAGGTGTCTCTGTTTTATGAGCAATTGAAGGGCTGGCGTGAGTTTGCCCGTCAGCAATCACTTTCAGATTTAATTTGG
AAAGTGTATCGTGAAACAGGGTATTATGACTTTGTTGGTGGTCTTCCGGCGGGAAAACAAAGACAAGCAAACTTACGTGT
ACTATATGACCGTGCTCGTCAATTTGAAGCAACATCATTCCGCGGATTATTCCGTTTCCTGCGCTTTATTGAACGTATTT
TAGAGCGTGGTGATGATATGGGTACAGCAAGGGCACTTGGCGAGCAAGAAGATGTCGTTCGCATTATGACGATTCATAAA
AGTAAAGGACTTGAATTCCCAGTTGTCTTTGTTGCGGGACTTGGTCGTAAATTTAATACGCAAGATTTAATGAAGCGCTT
CTTGCTTCATAAAGATTTAGGGTTCGGCTCGCAGTTTATTGATCCGCAAAAACGAATTAAATATACGACGCTCTCACAGC
TTGCGATTAAGCGAAAGATGAAAATGGAACTAATCGCTGAAGAGATGCGCGTATTATATGTTGCATTAACACGAGCGAAG
GAAAAGCTTATTTTAATCGGAACGGTAAAAGGAAAAGGGAAAGAGATGGAGAAGTGGCTCGAGGCAAGAGACCATGCCCA
GTGGCTATTGCCAGATTACGTGCGCGCTGGAGCTACTTGTTATTTAGATTGGATTGCCCCTTCTTTATTTAGGCATGGTG
ATAGTGAATCATTATTAGAGTTAGGGCAAGGGCTAATTCCTGATGATATTTATCGTTACGGAGCAAGTTTTAAAGTAGAA
GCGATTGATGGGTCTACTTTACAAGCCCCTGTTTTATTAAAAGAAGAGAAGCGAGATTTATTAGAAGCGCTTCGCGCAAA
AGAGCCAGTTTCTCTTGAAAGTGAGCAGAAGGAAGACGTATATGATAAGTTAACGTGGCAATATGAATTCCGTGATGCAA
CAGTCCATAGAGCGAAACAATCCGTTACGGAAATTAAACGAAACTATCAAGTGCAAGAAGGAAGCGATGGTTCGTTTATA
AAAAAACTTCGTGCTCCGATACAAGCGCGCCCACGTTTTATGGAGAAGAAAGGGTTAACATATGCAGAGCGCGGAACAGC
AGTGCATGCGGTTATGCAGTATGTTGATTTGAAAAAACCAATCACAGTGGAGAGCGTGGCAGAACAAATTGTTGAAATGG
TTAACAAAGAACTGTTAACATATGAGCAAGCAGAAGAAATACAAGCTGAAAAAATCGTGTCCTTCTTTGAAACAGAACTT
GGAAGACGACTAGTCGCTGCAAAAAGTGTAGAGCGTGAAGTTCCCTTTACAATGATGTTACCTGCTGAAGAGGCATATCA
AGATTGGAGCGGTAGCCCTGGTGAATCTGTACTTGTTCAAGGTGTCATTGATTGTATGGTGGAAGAAGAGGATGGTATTG
TGTTAATCGACTTTAAAACAGATACAATTGAAGGCCGCTTCCCTGGCGGATTTGAACAAGCGAGGCCAGTGTTAGAAGAA
CGCTACCATGTTCAGCTTTCGCTATACAGAAAGGCGCTCGAAAAAAGTTTACATCAACCAGTGAGGGAGACATATTTGTA
TTTCTTTGATGGTAGTCACGTATTGAAAATTTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-888)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1058-1228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.13

100

0.535

Multiple sequence alignment