Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AB8E30_RS00405 Genome accession   NZ_CP165965
Coordinates   57737..59113 (-) Length   458 a.a.
NCBI ID   WP_369901511.1    Uniprot ID   -
Organism   Bacillus manliponensis strain jeju3-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 52737..64113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8E30_RS00385 (AB8E30_00385) ispF 54106..54582 (-) 477 WP_369903403.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  AB8E30_RS00390 (AB8E30_00390) ispD 54702..55379 (-) 678 WP_369901509.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AB8E30_RS00395 (AB8E30_00395) - 55396..56501 (-) 1106 Protein_66 PIN/TRAM domain-containing protein -
  AB8E30_RS00400 (AB8E30_00400) disA 56660..57733 (-) 1074 WP_369901510.1 DNA integrity scanning diadenylate cyclase DisA -
  AB8E30_RS00405 (AB8E30_00405) radA 57737..59113 (-) 1377 WP_369901511.1 DNA repair protein RadA Machinery gene
  AB8E30_RS00410 (AB8E30_00410) clpC 59209..61644 (-) 2436 WP_034642662.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  AB8E30_RS00415 (AB8E30_00415) - 61658..62731 (-) 1074 WP_369901512.1 protein arginine kinase -
  AB8E30_RS00420 (AB8E30_00420) - 62736..63284 (-) 549 WP_369901513.1 UvrB/UvrC motif-containing protein -
  AB8E30_RS00425 (AB8E30_00425) - 63382..63843 (-) 462 WP_034642670.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50194.84 Da        Isoelectric Point: 7.1417

>NTDB_id=1032612 AB8E30_RS00405 WP_369901511.1 57737..59113(-) (radA) [Bacillus manliponensis strain jeju3-1]
MAKKKTKFVCQECGYHSPKYMGKCPGCGQWNTLTEEMEQVVSSRRINYANAIQTELTKPRRLKEVETKMEDRIETSFQEF
NRVLGGGIVNGSLVLIGGDPGIGKSTLLLQVSSQLADRSYDVLYISGEESAKQIKLRADRLHVNGDNLFVVSETDLQRIA
AYIDEMNPSFVVIDSIQTIYLPEVTSAPGSVSQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLHEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRAGLLLQNQDAYLKVAGGMKLDEPAVDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEVRRVSRIEQRVQEAAKLGFQRVIIPRKNLGGWTIPEGIEVVGVSNIGEALRVTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=1032612 AB8E30_RS00405 WP_369901511.1 57737..59113(-) (radA) [Bacillus manliponensis strain jeju3-1]
ATGGCTAAAAAGAAAACAAAATTTGTATGTCAAGAGTGTGGATATCATTCGCCAAAGTATATGGGAAAATGTCCAGGTTG
TGGTCAGTGGAATACATTAACGGAAGAAATGGAACAAGTTGTATCATCAAGACGTATTAATTATGCAAATGCAATTCAAA
CAGAGCTTACGAAACCTCGTCGTTTAAAAGAAGTAGAAACAAAAATGGAAGATCGTATTGAGACGAGCTTTCAAGAATTT
AATCGTGTACTTGGTGGTGGGATTGTAAATGGATCTCTCGTACTAATTGGAGGAGACCCTGGAATTGGGAAGTCTACTTT
GCTATTACAAGTATCATCTCAGTTAGCAGATCGTTCATATGATGTATTATATATATCTGGTGAAGAATCAGCGAAACAAA
TTAAGTTAAGGGCGGATCGCCTTCATGTAAATGGAGACAACTTATTTGTTGTTTCTGAGACGGATTTACAGCGAATTGCA
GCATATATAGATGAAATGAACCCATCCTTTGTTGTCATTGACTCGATTCAAACAATTTATTTACCTGAGGTGACTTCAGC
ACCTGGTAGTGTATCGCAAGTGCGAGAATGTACAGCCGAATTAATGAAACTAGCAAAAACAAAAGGTATACCTATTTTTA
TTGTTGGGCATGTAACAAAAGAAGGTGCAATTGCAGGGCCACGTATGTTAGAGCATATGGTAGATGCGGTTCTTTATTTT
GAAGGGGATCGTCATCACACATATCGTATTTTACGTGCTGTGAAAAACCGCTTTGGTTCTACAAATGAAATGGGCATTTT
TGAAATGAAGGAACTTGGTTTGCATGAAGTGTTAAATCCTTCGGAAATCTTTTTAGAAGAAAGACCAGTTGGCGTAGCGG
GTTCTACTGTTGTAGCTTCTATGGAGGGAACAAGACCGGTTTTAGTTGAAATTCAAGCGCTCATTTCACCTACAAGTTTT
GGGAATCCAAGAAGAATGGCTACAGGGATTGATCATAACCGAGTTTCATTAATTATGGCAGTACTCGAGAAGCGAGCGGG
GTTATTATTACAAAACCAGGACGCGTATTTAAAAGTTGCTGGTGGTATGAAGCTTGATGAACCAGCAGTTGATTTAGCGG
TTGCGTTAAGTATTGCGTCAAGCTTTCGTGATAAGTCAACAGCTCCAACAGATGCAGTGATTGGAGAAGTTGGTTTAACA
GGGGAAGTTCGAAGGGTATCTCGAATAGAACAGCGTGTGCAAGAAGCAGCAAAACTAGGTTTTCAACGAGTTATTATTCC
GAGAAAGAATCTAGGAGGCTGGACGATTCCTGAGGGGATTGAGGTAGTGGGTGTTTCAAATATAGGGGAGGCACTTCGAG
TAACGTTAGGAGGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(59-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.163

100

0.753
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629

Multiple sequence alignment