Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MFPB19_RS02685 Genome accession   NZ_LT960784
Coordinates   541971..543308 (+) Length   445 a.a.
NCBI ID   WP_056948838.1    Uniprot ID   -
Organism   Latilactobacillus sakei isolate MFPB19     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536971..548308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFPB19_RS02670 (MFPB19_0555) rny 538412..539977 (+) 1566 WP_076632200.1 ribonuclease Y -
  MFPB19_RS02675 (MFPB19_0556) - 540130..541218 (+) 1089 WP_056948881.1 glycosyltransferase family 4 protein -
  MFPB19_RS02680 (MFPB19_0557) - 541244..541909 (-) 666 WP_056948841.1 YigZ family protein -
  MFPB19_RS02685 (MFPB19_0558) comFA 541971..543308 (+) 1338 WP_056948838.1 DEAD/DEAH box helicase Machinery gene
  MFPB19_RS02690 (MFPB19_0559) comFC 543284..543979 (+) 696 WP_147658291.1 ComF family protein Machinery gene
  MFPB19_RS02695 (MFPB19_0560) hpf 544106..544651 (+) 546 WP_056948836.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MFPB19_RS02700 (MFPB19_0561) secA 544900..547263 (+) 2364 WP_011374203.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 50226.69 Da        Isoelectric Point: 9.6659

>NTDB_id=1032425 MFPB19_RS02685 WP_056948838.1 541971..543308(+) (comFA) [Latilactobacillus sakei isolate MFPB19]
MGQPVIACGRQFTAAQLADTQNNNYSLPQIERRPAFLRVKQRLICQRCQQVVPSQTCLPDGRHYCAQCLLFGRLVEGDWL
YTIPEDHLFKTATPKLTWAGQLTVYQEQAAQAVVAVIQAHKWHVLTAVTGAGKTEMLFQGILVALQKGQRVCLAAPRVAV
CLELYPRLQAAFATTSIMLMHGEQTEPYRYTQLVICTTHQLLKFYHAFDTVIVDEVDAFPFVDNPVLATAVEQACKPQCA
LLYLTATPTPAIKRAIAAKQMTVSELPLRFHGGILPEPQRHVAFNWRPRLKKGRLPKRLEHDCQICLKNQQILLFVPQVR
LLKPVANRLSILLPAVRIETVHANDPEQIEKIAAFRAQQIQLMVTTTILERGVTFKNVAVLVLGAEHMVFNEAVLVQIAG
RAGRHKDYTQNPVHFYYQDYTRAIKGACRQIKAQNQRGWRLQSTV

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=1032425 MFPB19_RS02685 WP_056948838.1 541971..543308(+) (comFA) [Latilactobacillus sakei isolate MFPB19]
ATGGGACAACCAGTGATTGCCTGTGGCCGCCAGTTTACTGCGGCCCAGTTAGCCGATACGCAAAATAATAATTATAGTTT
ACCACAGATTGAGCGCCGACCCGCGTTTTTACGGGTCAAGCAACGTTTAATCTGCCAACGTTGTCAGCAAGTGGTACCGT
CGCAAACTTGCTTACCAGACGGCCGTCATTATTGCGCGCAGTGTTTGTTATTTGGTCGTTTAGTAGAAGGGGATTGGTTA
TATACAATCCCTGAGGATCATTTATTTAAAACCGCTACACCGAAGTTGACGTGGGCAGGCCAATTAACAGTGTATCAGGA
ACAAGCCGCCCAAGCAGTGGTAGCGGTTATTCAAGCCCATAAATGGCACGTATTAACAGCCGTGACCGGTGCCGGGAAGA
CCGAGATGCTTTTTCAAGGGATTCTAGTCGCACTACAAAAGGGACAGCGAGTGTGTTTGGCAGCACCTAGAGTCGCAGTT
TGTCTAGAACTGTATCCGCGCCTGCAAGCCGCCTTTGCGACGACTAGTATCATGTTGATGCATGGCGAGCAAACCGAGCC
CTATCGCTATACCCAGTTAGTGATTTGCACGACACATCAATTACTGAAGTTTTATCACGCTTTTGATACGGTCATCGTTG
ATGAAGTGGATGCTTTTCCTTTTGTTGATAATCCAGTTTTAGCCACGGCAGTTGAACAAGCCTGTAAGCCTCAGTGTGCG
TTATTATATTTAACGGCTACGCCGACACCAGCCATCAAGCGGGCGATTGCTGCTAAACAAATGACAGTTAGTGAATTACC
GCTTCGCTTTCATGGGGGAATATTGCCAGAACCGCAACGGCATGTCGCTTTCAATTGGCGGCCGCGATTAAAGAAGGGCC
GGTTACCTAAACGACTTGAGCATGATTGCCAGATTTGTTTAAAAAACCAGCAGATTTTGCTATTTGTACCGCAAGTCAGA
TTATTAAAACCAGTTGCCAATCGACTATCAATTTTATTACCGGCGGTCAGAATTGAGACGGTGCACGCAAACGATCCTGA
ACAAATTGAAAAGATCGCGGCGTTTAGGGCTCAACAAATTCAATTAATGGTGACGACCACTATTTTGGAGCGGGGGGTCA
CATTTAAAAATGTTGCGGTTTTGGTCTTAGGCGCGGAACACATGGTGTTTAATGAAGCCGTTTTGGTCCAGATTGCCGGG
CGAGCAGGACGGCATAAAGACTATACCCAAAATCCGGTTCATTTTTATTATCAGGATTATACCCGCGCTATTAAAGGAGC
TTGTCGGCAAATTAAGGCCCAAAATCAGAGGGGATGGCGATTACAATCAACTGTTTAA

Domains

Predicted by InterProScan.

Helicase conserved C-terminal domain (Helicase_C)

(307-405)

Type III restriction enzyme, res subunit (ResIII)

(102-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

91.685

100

0.917
  comFA/cflA Streptococcus pneumoniae TIGR4

38.979

96.854

0.378
  comFA/cflA Streptococcus pneumoniae Rx1

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae D39

38.747

96.854

0.375
  comFA/cflA Streptococcus pneumoniae R6

38.747

96.854

0.375
  comFA/cflA Streptococcus mitis NCTC 12261

38.515

96.854

0.373
  comFA/cflA Streptococcus mitis SK321

38.372

96.629

0.371