Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AB3D65_RS15010 Genome accession   NZ_CP165606
Coordinates   2974767..2975204 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain BP1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2969767..2980204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3D65_RS14995 (AB3D65_14995) - 2972625..2972987 (-) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -
  AB3D65_RS15000 (AB3D65_15000) hxlA 2973219..2973854 (+) 636 WP_014416875.1 3-hexulose-6-phosphate synthase -
  AB3D65_RS15005 (AB3D65_15005) hxlB 2973851..2974408 (+) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  AB3D65_RS15010 (AB3D65_15010) nucA/comI 2974767..2975204 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AB3D65_RS15015 (AB3D65_15015) nin/comJ 2975225..2975623 (+) 399 WP_014416873.1 competence protein ComJ Regulator
  AB3D65_RS15020 (AB3D65_15020) - 2975664..2977100 (-) 1437 WP_369547143.1 glycoside hydrolase family 1 protein -
  AB3D65_RS15025 (AB3D65_15025) - 2977204..2977482 (-) 279 WP_263612330.1 YckD family protein -
  AB3D65_RS15030 (AB3D65_15030) - 2977599..2978033 (-) 435 WP_014416871.1 RDD family protein -
  AB3D65_RS15035 (AB3D65_15035) - 2978048..2979184 (-) 1137 WP_369547144.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=1030822 AB3D65_RS15010 WP_003156588.1 2974767..2975204(+) (nucA/comI) [Bacillus velezensis strain BP1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1030822 AB3D65_RS15010 WP_003156588.1 2974767..2975204(+) (nucA/comI) [Bacillus velezensis strain BP1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATACATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment