Detailed information NTG1030161

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB5S07_RS09920
• Genome accession: NZ_CP163505
• Coordinates: 2204373..2205167 (-)
• Length: 264 a.a.
• NCBI ID: WP_038244550.1
• Uniprot ID: A0A085U423
• Organism: Yersinia ruckeri strain Y2022-239
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2199373..2210167

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB5S07_RS09900 (AB5S07_09900)	-	2200019..2201169 (+)	1151	WP_115175277.1	IS3 family transposase	-
AB5S07_RS09905 (AB5S07_09905)	-	2201185..2201943 (-)	759	WP_230596561.1	PepSY domain-containing protein	-
AB5S07_RS09910 (AB5S07_09910)	gbpA	2202063..2203553 (-)	1491	WP_004722689.1	N-acetylglucosamine-binding protein GbpA	-
AB5S07_RS09915 (AB5S07_09915)	gspS	2203979..2204353 (+)	375	WP_004722686.1	type II secretion system pilot lipoprotein GspS	-
AB5S07_RS09920 (AB5S07_09920)	pilD	2204373..2205167 (-)	795	WP_038244550.1	A24 family peptidase	Machinery gene
AB5S07_RS09925 (AB5S07_09925)	gspM	2205164..2205661 (-)	498	WP_230596562.1	type II secretion system protein GspM	-
AB5S07_RS09930 (AB5S07_09930)	gspL	2205645..2206844 (-)	1200	WP_004722681.1	type II secretion system protein GspL	-
AB5S07_RS09935 (AB5S07_09935)	gspK	2206846..2207931 (-)	1086	WP_230596563.1	type II secretion system minor pseudopilin GspK	-
AB5S07_RS09940 (AB5S07_09940)	gspJ	2207921..2208526 (-)	606	WP_038251265.1	type II secretion system minor pseudopilin GspJ	-
AB5S07_RS09945 (AB5S07_09945)	gspI	2208523..2208885 (-)	363	WP_038244547.1	type II secretion system minor pseudopilin GspI	-
AB5S07_RS09950 (AB5S07_09950)	gspH	2208882..2209481 (-)	600	WP_045844477.1	type II secretion system minor pseudopilin GspH	-
AB5S07_RS09955 (AB5S07_09955)	gspG	2209481..2209948 (-)	468	WP_004722674.1	type II secretion system major pseudopilin GspG	-

Sequence

Protein

• Length: 264 a.a.
• Molecular weight: 29761.04 Da
• Isoelectric Point: 8.4833

>NTDB_id=1030161 AB5S07_RS09920 WP_038244550.1 2204373..2205167(-) (pilD) [Yersinia ruckeri strain Y2022-239]
MMDALYMAQMIAPLWWGGLAVLGLCVGSFLNVAIYRLPLMLTRENPHGFNLCLPHSHCPQCKHSLKWRDNIPVFSWLFLQ
GQCRYCRASIPVRYPLVEIMTMLMTLFIAAVIPLGSPLLAALLLSWMLIALTMIDIDHLLLPDNLTLPLLWMGLLFHLFD
SRLALSEAVIGAVAGYLILWCLYWSFWLLTKREALGYGDFKLLAALGAWLGWMALPSLLLIASLCGMSFALAARLLRGRP
LNTALPFGPFLAFGGWILFLQYYR

Nucleotide

• Length: 795 bp

>NTDB_id=1030161 AB5S07_RS09920 WP_038244550.1 2204373..2205167(-) (pilD) [Yersinia ruckeri strain Y2022-239]
ATGATGGATGCGCTCTATATGGCTCAAATGATAGCGCCACTGTGGTGGGGCGGGCTGGCGGTTCTTGGCCTGTGTGTCGG
TAGTTTTCTCAACGTCGCTATTTATCGGCTACCGCTGATGTTAACCCGCGAAAATCCGCATGGTTTTAATCTGTGTCTAC
CTCATTCCCACTGTCCTCAGTGTAAACATTCGTTGAAATGGCGAGATAATATTCCTGTATTCAGTTGGCTATTCCTGCAA
GGACAATGTCGTTACTGTCGGGCATCTATCCCAGTGCGCTACCCGCTGGTTGAAATCATGACGATGTTGATGACCTTGTT
TATTGCTGCAGTCATCCCATTGGGCAGCCCGTTACTGGCCGCACTACTACTCAGTTGGATGCTGATCGCGTTAACCATGA
TCGATATTGACCACTTGCTCTTACCGGATAATCTGACATTGCCTCTGCTATGGATGGGGCTACTTTTTCACCTGTTTGAT
AGTCGGTTGGCATTAAGTGAGGCAGTGATTGGTGCTGTCGCAGGATATTTGATCTTATGGTGCCTTTACTGGTCGTTCTG
GCTGTTGACGAAGCGGGAAGCTCTGGGCTACGGAGACTTTAAGTTATTAGCCGCATTAGGTGCCTGGCTAGGATGGATGG
CGTTACCCTCTTTACTTTTGATCGCCTCGCTCTGCGGCATGAGTTTCGCACTGGCCGCACGTCTCCTACGGGGCAGACCA
CTGAATACGGCACTGCCATTCGGGCCGTTTCTCGCATTCGGTGGCTGGATTCTATTCCTTCAGTACTACCGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-230)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-110)

3D structure

• AlphaFold DB: A0A085U423

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.181	96.212	0.492
pilD	Vibrio campbellii strain DS40M4	46.667	96.591	0.451
pilD	Neisseria gonorrhoeae MS11	44.531	96.97	0.432
pilD	Acinetobacter baumannii D1279779	42.471	98.106	0.417
pilD	Acinetobacter nosocomialis M2	42.085	98.106	0.413