Detailed information NTG1028364

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB4O99_RS23710
• Genome accession: NZ_CP162997
• Coordinates: 5004153..5004953 (+)
• Length: 266 a.a.
• NCBI ID: WP_087828446.1
• Uniprot ID: -
• Organism: Klebsiella pasteurii strain W257
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4999153..5009953

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB4O99_RS23675 (AB4O99_23675)	gspH	4999294..4999806 (+)	513	WP_311123534.1	type II secretion system minor pseudopilin GspH	-
AB4O99_RS23680 (AB4O99_23680)	gspI	4999803..5000168 (+)	366	WP_049115511.1	type II secretion system minor pseudopilin GspI	-
AB4O99_RS23685 (AB4O99_23685)	gspJ	5000165..5000761 (+)	597	WP_311123535.1	type II secretion system minor pseudopilin GspJ	-
AB4O99_RS23690 (AB4O99_23690)	gspK	5000758..5001738 (+)	981	WP_311123536.1	type II secretion system minor pseudopilin GspK	-
AB4O99_RS23695 (AB4O99_23695)	gspL	5001731..5002927 (+)	1197	WP_311123537.1	type II secretion system protein GspL	-
AB4O99_RS23700 (AB4O99_23700)	-	5002920..5003405 (+)	486	WP_311123538.1	type II secretion system protein M	-
AB4O99_RS23705 (AB4O99_23705)	-	5003395..5004153 (+)	759	WP_224326399.1	type II secretion system protein N	-
AB4O99_RS23710 (AB4O99_23710)	pilD	5004153..5004953 (+)	801	WP_087828446.1	A24 family peptidase	Machinery gene
AB4O99_RS23715 (AB4O99_23715)	hrpB	5005051..5007480 (-)	2430	WP_142471715.1	ATP-dependent helicase HrpB	-
AB4O99_RS23720 (AB4O99_23720)	thpR	5007553..5008089 (+)	537	WP_004129056.1	RNA 2',3'-cyclic phosphodiesterase	-
AB4O99_RS23725 (AB4O99_23725)	sfsA	5008089..5008805 (+)	717	WP_004129055.1	DNA/RNA nuclease SfsA	-
AB4O99_RS23730 (AB4O99_23730)	dksA	5008970..5009425 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29071.64 Da
• Isoelectric Point: 7.0576

>NTDB_id=1028364 AB4O99_RS23710 WP_087828446.1 5004153..5004953(+) (pilD) [Klebsiella pasteurii strain W257]
MFENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMAHAEGINLCFPASFCPQCREPIAWRNNIPLLSFLF
LKGRSRCCGEPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLLLLSVLLILATIDAQTQLLPDGLTLPLLWAGLLFNL
SDTFAPLAEAVIGAMAGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQALPQTLLLASASGLVWTLLQRLITR
QSFQQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 801 bp

>NTDB_id=1028364 AB4O99_RS23710 WP_087828446.1 5004153..5004953(+) (pilD) [Klebsiella pasteurii strain W257]
ATGTTCGAAAACATCGCGTTATTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGG
ACTGGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGCGCACGCAGAGGGAATCAATCTCT
GTTTTCCCGCGTCGTTCTGTCCGCAGTGCCGTGAGCCCATCGCCTGGCGGAACAATATCCCGCTGCTGAGCTTCCTGTTT
CTTAAGGGACGTTCGCGCTGCTGCGGGGAACCCATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTT
TATGCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGCTTGCTTCTGCTGTCGGTTCTACTGATTCTTG
CTACCATCGACGCGCAAACGCAGCTGCTGCCGGACGGGTTGACGCTACCGCTGCTGTGGGCCGGTCTGCTGTTCAATCTT
TCCGATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGCCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGT
GTTCCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCGGCGCTCGGCGCGTGGCTCGGCT
GGCAGGCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGCCTGGTCTGGACGCTGCTGCAGCGCCTGATAACCCGG
CAGTCGTTCCAGCAGCCTTTGGCCTTCGGGCCTTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATA
A

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (124-232)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (26-111)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.496
pilD	Neisseria gonorrhoeae MS11	47.431	95.113	0.451
pilD	Vibrio campbellii strain DS40M4	42.545	100	0.44
pilD	Acinetobacter nosocomialis M2	44.015	97.368	0.429
pilD	Acinetobacter baumannii D1279779	43.629	97.368	0.425