Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   AB4L27_RS07870 Genome accession   NZ_CP162907
Coordinates   1685334..1686350 (-) Length   338 a.a.
NCBI ID   WP_005395718.1    Uniprot ID   A0AAE4S9C7
Organism   Vibrio diabolicus strain 3098     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1680334..1691350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4L27_RS07845 (AB4L27_07845) aroK 1681204..1681722 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AB4L27_RS07850 (AB4L27_07850) pilQ 1681910..1683658 (-) 1749 WP_006742777.1 type IV pilus secretin PilQ Machinery gene
  AB4L27_RS07855 (AB4L27_07855) pilP 1683684..1684199 (-) 516 WP_237312731.1 pilus assembly protein PilP Machinery gene
  AB4L27_RS07860 (AB4L27_07860) pilO 1684189..1684782 (-) 594 WP_012841351.1 type 4a pilus biogenesis protein PilO Machinery gene
  AB4L27_RS07865 (AB4L27_07865) pilN 1684775..1685350 (-) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  AB4L27_RS07870 (AB4L27_07870) pilM 1685334..1686350 (-) 1017 WP_005395718.1 type IV pilus biogenesis protein PilM Machinery gene
  AB4L27_RS07875 (AB4L27_07875) - 1686494..1689055 (+) 2562 WP_256935792.1 penicillin-binding protein 1A -
  AB4L27_RS07880 (AB4L27_07880) oxyR 1689107..1690018 (-) 912 WP_005395720.1 DNA-binding transcriptional regulator OxyR -
  AB4L27_RS07885 (AB4L27_07885) - 1690189..1690917 (+) 729 WP_005395721.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.08 Da        Isoelectric Point: 6.9711

>NTDB_id=1027977 AB4L27_RS07870 WP_005395718.1 1685334..1686350(-) (pilM) [Vibrio diabolicus strain 3098]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1027977 AB4L27_RS07870 WP_005395718.1 1685334..1686350(-) (pilM) [Vibrio diabolicus strain 3098]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAATTGCAAATAGAACAAAACCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCATTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGTGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTTCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAAATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment