Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB4P93_RS23295 Genome accession   NZ_CP162596
Coordinates   4899253..4901328 (+) Length   691 a.a.
NCBI ID   WP_395593313.1    Uniprot ID   -
Organism   Pseudomonas sp. B26140     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4894253..4906328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P93_RS23275 (AB4P93_23225) pilM 4896465..4897529 (+) 1065 WP_210702919.1 pilus assembly protein PilM Machinery gene
  AB4P93_RS23280 (AB4P93_23230) - 4897529..4898095 (+) 567 WP_150593940.1 PilN domain-containing protein -
  AB4P93_RS23285 (AB4P93_23235) pilO 4898092..4898715 (+) 624 WP_210702920.1 type 4a pilus biogenesis protein PilO -
  AB4P93_RS23290 (AB4P93_23240) - 4898712..4899239 (+) 528 WP_210702921.1 pilus assembly protein PilP -
  AB4P93_RS23295 (AB4P93_23245) pilQ 4899253..4901328 (+) 2076 WP_395593313.1 type IV pilus secretin PilQ Machinery gene
  AB4P93_RS23300 (AB4P93_23250) aroK 4901333..4901851 (+) 519 WP_042557267.1 shikimate kinase AroK -
  AB4P93_RS23305 (AB4P93_23255) aroB 4902003..4903103 (+) 1101 WP_210702923.1 3-dehydroquinate synthase -
  AB4P93_RS23310 (AB4P93_23260) - 4903114..4904706 (+) 1593 WP_395593314.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74971.45 Da        Isoelectric Point: 5.5789

>NTDB_id=1027353 AB4P93_RS23295 WP_395593313.1 4899253..4901328(+) (pilQ) [Pseudomonas sp. B26140]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYDTRVEGNYLFVVVGQGAPAKAPRPAVAAPRVATTAAPAKAFVAKSRAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAGVTGDRTVITVEPSGT
FDYSTFQTDNKLTVSIRPMTFDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
IDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVNG
SSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=1027353 AB4P93_RS23295 WP_395593313.1 4899253..4901328(+) (pilQ) [Pseudomonas sp. B26140]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGTTGTCACCGATGGTACAGGCGGCCAACTTGAA
GGCGCTGGATGTGGCAGCGTTGCCGGGTGATCGAGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCGCCGCAGCCCAAGG
GTTACACCACCGAGTCACCGGCGCGAATTGCGCTGGACTTGCCCGGTGTGGCCAGTCAGTTGGCGAGCAAAAATCTCGAT
CTGGGCAGTGGCAATGCGCGCACGGCCACGGTGGTTGAAGCCAAGGACCGCACGCGGCTGATTGTCAGTCTCACGCAATT
GGCGCCTTACGACACACGGGTTGAGGGCAACTATCTGTTCGTGGTGGTTGGTCAGGGCGCGCCGGCCAAGGCGCCGCGAC
CGGCAGTGGCGGCACCACGCGTGGCGACTACTGCTGCCCCCGCCAAGGCGTTCGTGGCGAAATCCCGCGCCATTCGCGGT
GTGGATTTTCAGCGCGGCACTGCGGGCGAGGGCAATGTCGTCATCGACCTGTCCGACCCGACCATCGCCCCGGATATCCA
GGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGTACGCAATTGCCGGAAAAGCTGCGGGTGCGTCTCGACGTGAAGG
ATTTCGCCACGCCAGTGCAATTCGTCAACGCCGGCGTAACCGGTGATCGCACGGTCATTACCGTCGAGCCCAGCGGCACC
TTTGACTATTCCACGTTCCAGACCGATAACAAACTGACCGTCAGCATCCGTCCGATGACCTTCGATGACCTGCAAAAACG
CAACGCCGATCGTCAGGCCTATGTCGGTGAAAAGCTCTCGTTGAACTTCCAGGACATTGACGTGCGCTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTGGCGAGCGACACGGTGCAGGGCGGCATCACCCTGCGTTTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTGAAGACCAAAGGCCTGGATAAACGCAAAATCGGTAATGTGCTGCTGGTGGC
CCCGGCTGATGAAATTGCCGCGCGCGAACGTCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGCTGCGTC
GTGAGCTGTTGCAGGTGAACTATGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAG
ATCGATGAGCGTGGGTCAATCACTGTCGACGAACGGACCAACAACATCATTGCCTACCAGACTCAGGATCGCCTCGACGA
GCTGCGGCGGATCGTGGCGCAACTGGATATTCCAGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACGTCG
ATTACGACAAGAGTCTGGGGGTGCGCTGGGGTGGTTCGATCCAGAACAAGGGCAACTGGAATGCCTCCGGGGTCAACGGT
TCATCGACCACCATCGGCACGCCGGGCAGCACCAGTACCAACTCGCCGTTCGTCGACATGGGGACCGTCGGCAATACTTC
CGGGATCGGTATCGCGTTCATTACCGACAACGTTTTGCTCGATCTGGAGCTGACGGCCATGGAGAAGACCGGCAACGGTG
AAATCGTTTCGCAGCCCAAAGTGGTCACCTCCGACAAAGAGACCGCGAAGATCCTCAAAGGCACCGAGATTCCTTACCAG
GAAGCCAGCTCCAGCGGCGCCACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACCCCGCAGATCACCCC
GGATAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAAAAGAACGAGGTCAACGCCAAAGTGCTGGTCAATGATGGCGAAACCATCGTGATTGGGGGGGTTTTCTCAAATACT
CAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(526-682)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.753
  comQ Acinetobacter baylyi ADP1

41.126

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.121

100

0.411
  pilQ Acinetobacter baumannii D1279779

38.121

100

0.411
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.829

0.373
  pilQ Legionella pneumophila strain Lp02

38.166

97.829

0.373

Multiple sequence alignment