Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB4J90_RS13575 Genome accession   NZ_CP162496
Coordinates   2659658..2660728 (-) Length   356 a.a.
NCBI ID   WP_029760727.1    Uniprot ID   -
Organism   Geobacillus thermodenitrificans strain BIM B-1973     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2654658..2665728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4J90_RS13535 (AB4J90_13535) - 2655491..2656285 (+) 795 WP_011887796.1 YqhG family protein -
  AB4J90_RS13540 (AB4J90_13540) - 2656362..2656541 (-) 180 WP_008879845.1 YqzE family protein -
  AB4J90_RS13545 (AB4J90_13545) comGG 2656587..2656976 (-) 390 WP_008879846.1 competence type IV pilus minor pilin ComGG -
  AB4J90_RS13550 (AB4J90_13550) comGF 2657110..2657595 (-) 486 WP_011887798.1 competence type IV pilus minor pilin ComGF -
  AB4J90_RS13555 (AB4J90_13555) - 2657532..2657858 (-) 327 WP_011887799.1 hypothetical protein -
  AB4J90_RS13560 (AB4J90_13560) comGD 2657842..2658279 (-) 438 WP_008879849.1 competence type IV pilus minor pilin ComGD -
  AB4J90_RS13565 (AB4J90_13565) comGC 2658269..2658565 (-) 297 WP_011887800.1 competence type IV pilus major pilin ComGC Machinery gene
  AB4J90_RS13570 (AB4J90_13570) comGB 2658633..2659661 (-) 1029 WP_011887801.1 competence type IV pilus assembly protein ComGB -
  AB4J90_RS13575 (AB4J90_13575) comGA 2659658..2660728 (-) 1071 WP_029760727.1 competence type IV pilus ATPase ComGA Machinery gene
  AB4J90_RS13580 (AB4J90_13580) - 2660928..2661626 (+) 699 WP_029760726.1 metalloregulator ArsR/SmtB family transcription factor -
  AB4J90_RS13585 (AB4J90_13585) - 2661790..2662032 (+) 243 WP_008879854.1 DUF2626 family protein -
  AB4J90_RS13590 (AB4J90_13590) - 2662063..2663190 (-) 1128 WP_011887804.1 class I SAM-dependent methyltransferase -
  AB4J90_RS13595 (AB4J90_13595) - 2663422..2664054 (-) 633 WP_008879856.1 MBL fold metallo-hydrolase -
  AB4J90_RS13600 (AB4J90_13600) - 2664234..2664410 (+) 177 WP_008879857.1 DUF2759 domain-containing protein -
  AB4J90_RS13605 (AB4J90_13605) - 2664446..2665609 (-) 1164 WP_008879858.1 M14 family metallocarboxypeptidase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39595.73 Da        Isoelectric Point: 8.6314

>NTDB_id=1026529 AB4J90_RS13575 WP_029760727.1 2659658..2660728(-) (comGA) [Geobacillus thermodenitrificans strain BIM B-1973]
MDDIEQVANRLLAEAVQRRASDLHLVPRRHDAIIRLRLDGMLIDVGTISKETAERVIVHFKFLAGMDIGERRRPQSGAME
VNESGETVYLRLSTLPTLYDESLVIRLLPQRFSLPLRELSLFSHSTARLFSFMQQPQGLVLLTGPTGSGKTTTLYTLLDV
CQAERQRNIITLEDPIEKRNDRFLQVQINEKAGITYAASLKAALRHDPDVLMVGEIRDHDTAAIAVRSALTGHLVVSTMH
AADAVGAVYRLHEFGVSPGDLAETLLAVSAQRLVELRCPLCGDNCHPACRRLQRRRRAAVHELLYGSALFDVIRSLSGEG
EQRLRPHLTLARLIRKGIALGYLPTRALELVGGGER

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1026529 AB4J90_RS13575 WP_029760727.1 2659658..2660728(-) (comGA) [Geobacillus thermodenitrificans strain BIM B-1973]
CTGGATGATATCGAACAAGTAGCGAACCGTCTCCTCGCCGAAGCGGTGCAACGCCGCGCCTCTGACCTTCATCTCGTCCC
GCGCCGACATGATGCGATCATTCGCCTCCGCCTTGACGGCATGCTCATCGATGTTGGTACAATCTCGAAAGAAACCGCTG
AACGCGTCATCGTCCATTTCAAATTTTTGGCTGGCATGGATATTGGCGAACGCCGTCGTCCGCAAAGCGGCGCGATGGAA
GTGAATGAGTCCGGGGAAACGGTGTATTTGCGCTTATCTACGCTGCCGACGCTTTATGATGAAAGCCTCGTCATCCGCCT
TCTACCGCAGCGCTTTTCGCTGCCGCTTCGTGAACTATCCTTATTTTCCCACTCCACCGCACGATTGTTTTCCTTTATGC
AGCAGCCACAAGGACTCGTGTTGCTGACCGGCCCAACCGGGTCGGGAAAGACGACGACATTGTATACGCTTCTCGACGTT
TGCCAAGCGGAAAGACAACGTAACATTATCACGTTGGAAGATCCGATTGAAAAACGGAATGATCGGTTTTTGCAAGTGCA
GATCAATGAAAAAGCAGGTATCACGTATGCAGCCAGCTTGAAAGCCGCTTTGCGCCATGACCCGGATGTGTTAATGGTCG
GCGAGATCCGCGACCATGATACGGCAGCGATCGCCGTACGATCAGCGTTGACAGGGCACTTGGTGGTATCGACCATGCAT
GCTGCTGATGCAGTCGGCGCAGTGTATCGGCTCCATGAGTTCGGGGTGTCTCCCGGCGACTTGGCTGAGACGTTGCTTGC
TGTTTCGGCGCAGCGTCTTGTTGAGCTTCGCTGCCCGCTATGCGGTGACAATTGCCATCCGGCTTGTCGTCGGCTTCAAC
GAAGACGGCGTGCTGCCGTTCATGAGTTGCTTTACGGCTCAGCGCTCTTTGATGTCATCCGTTCGCTGTCGGGGGAAGGC
GAGCAACGGCTCCGCCCACATCTGACATTAGCACGCCTCATCCGCAAAGGAATCGCACTCGGGTATTTGCCGACTCGCGC
GCTTGAACTTGTCGGAGGGGGAGAACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

51.585

97.472

0.503
  pilB Glaesserella parasuis strain SC1401

37.83

95.787

0.362

Multiple sequence alignment