Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   AB3454_RS12835 Genome accession   NZ_CP162418
Coordinates   2581763..2582449 (-) Length   228 a.a.
NCBI ID   WP_001793467.1    Uniprot ID   A0A641AAY3
Organism   Staphylococcus aureus strain TUM22750     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2576763..2587449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3454_RS12815 (AB3454_12815) rpsT 2577677..2577928 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  AB3454_RS12820 (AB3454_12820) holA 2577973..2578947 (-) 975 WP_001282568.1 DNA polymerase III subunit delta -
  AB3454_RS12825 (AB3454_12825) comEC 2579004..2581205 (-) 2202 WP_172576660.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  AB3454_RS12830 (AB3454_12830) - 2581210..2581671 (-) 462 WP_000439693.1 ComE operon protein 2 -
  AB3454_RS12835 (AB3454_12835) comEA 2581763..2582449 (-) 687 WP_001793467.1 ComEA family DNA-binding protein Machinery gene
  AB3454_RS12840 (AB3454_12840) - 2582489..2583205 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  AB3454_RS12845 (AB3454_12845) rsfS 2583208..2583561 (-) 354 WP_001088022.1 ribosome silencing factor -
  AB3454_RS12850 (AB3454_12850) yqeK 2583562..2584146 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  AB3454_RS12855 (AB3454_12855) nadD 2584136..2584705 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  AB3454_RS12860 (AB3454_12860) yhbY 2584708..2584998 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  AB3454_RS12865 (AB3454_12865) aroE 2585002..2585808 (-) 807 WP_000666748.1 shikimate dehydrogenase -
  AB3454_RS12870 (AB3454_12870) yqeH 2585822..2586922 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25786.50 Da        Isoelectric Point: 9.6753

>NTDB_id=1025133 AB3454_RS12835 WP_001793467.1 2581763..2582449(-) (comEA) [Staphylococcus aureus strain TUM22750]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDNALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1025133 AB3454_RS12835 WP_001793467.1 2581763..2582449(-) (comEA) [Staphylococcus aureus strain TUM22750]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATAATG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A641AAY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

100

100

1
  comEA Staphylococcus aureus N315

97.368

100

0.974

Multiple sequence alignment