Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ECRM12581_RS25285 Genome accession   NZ_CP007136
Coordinates   4925262..4927460 (-) Length   732 a.a.
NCBI ID   WP_001400571.1    Uniprot ID   -
Organism   Escherichia coli O145:H28 str. RM12581     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4920262..4932460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECRM12581_RS25265 (ECRM12581_24875) hslU 4921066..4922397 (-) 1332 WP_001293341.1 HslU--HslV peptidase ATPase subunit -
  ECRM12581_RS25270 (ECRM12581_24880) hslV 4922407..4922937 (-) 531 WP_000208242.1 ATP-dependent protease subunit HslV -
  ECRM12581_RS25275 (ECRM12581_24885) ftsN 4923030..4923989 (-) 960 WP_000068838.1 cell division protein FtsN -
  ECRM12581_RS25280 (ECRM12581_24890) cytR 4924081..4925106 (-) 1026 WP_000644904.1 DNA-binding transcriptional regulator CytR Regulator
  ECRM12581_RS25285 (ECRM12581_24900) priA 4925262..4927460 (-) 2199 WP_001400571.1 primosomal protein N' Machinery gene
  ECRM12581_RS25290 (ECRM12581_24905) rpmE 4927663..4927875 (+) 213 WP_000710769.1 50S ribosomal protein L31 -
  ECRM12581_RS25295 (ECRM12581_24910) yiiX 4927936..4928544 (-) 609 WP_000702305.1 YiiX family permuted papain-like enzyme -
  ECRM12581_RS25300 (ECRM12581_24915) metJ 4928604..4928921 (-) 318 WP_000852812.1 met regulon transcriptional regulator MetJ -
  ECRM12581_RS25305 (ECRM12581_24920) metB 4929198..4930358 (+) 1161 WP_001400572.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 732 a.a.        Molecular weight: 81630.93 Da        Isoelectric Point: 9.0294

>NTDB_id=102472 ECRM12581_RS25285 WP_001400571.1 4925262..4927460(-) (priA) [Escherichia coli O145:H28 str. RM12581]
MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIGVVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRL
LLWAADYYHHPIGDVLFHALPILLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFSAWLLAGVTGSGKTEVYLSVLEN
VLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVLHSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVID
EEHDSSYKQQEGWRYHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVLDLKGQKVQ
AGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDHYYTLHQAQQHLRCHHCDSQRPVPRQCPSCG
STHLVPVGLGTEQLEQTLAPLFPGVPISRIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVD
GALFSADFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAERRMMQLPPWTSHVIVR
AEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPKRGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRK
VKWVLDVDPIEG

Nucleotide


Download         Length: 2199 bp        

>NTDB_id=102472 ECRM12581_RS25285 WP_001400571.1 4925262..4927460(-) (priA) [Escherichia coli O145:H28 str. RM12581]
ATGCCCGTTGCCCACGTTGCCTTGCCCGTTCCGCTTCCTCGTACCTTTGACTATCTGCTGCCAGAAGGCATGACGGTTAA
AGCCGGGTGTCGCGTGCGCGTGCCGTTTGGCAAACAGCAGGAGCGCATCGGGGTTGTGGTATCAGTTAGCGATGTCAGTG
AGCTGCCGCTTAACGAGCTGAAAGCGGTAGTTGAAGTTCTGGACGTTGAGCCGGTATTTACTCACTCCGTCTGGCGATTA
TTGCTATGGGCGGCAGATTACTATCATCATCCGATTGGCGATGTGCTGTTTCATGCCTTGCCGATTTTACTGCGCCAGGG
GCGGCCTGCGGCGAACGCGCCGATGTGGTACTGGTTTGCCACTGAACAAGGCCAGGCGGTGGATCTGAACAGCCTGAAAC
GTTCGCCAAAGCAACAACAGGCACTGGCGGCATTGCGGCAAGGCAAAATCTGGCGCGACCAGGTTGCCACGCTTGAATTT
AATGATGCCGCGTTACAGGCGCTGCGCAAAAAAGGTCTGTGTGATTTAGCAAGTGAAACACCAGAGTTTAGCGACTGGCG
AACGAACTATGCCGTTTCTGGTGAACGGTTGCGATTGAATACCGAACAGGCCACCGCCGTTGGCGCAATTCATAGCGCGG
CGGATACCTTCTCTGCCTGGCTGCTGGCGGGCGTTACAGGTTCCGGTAAAACAGAGGTTTATTTGAGCGTACTGGAAAAC
GTGCTCGCTCAGGGCAAACAGGCGCTGGTGATGGTGCCGGAAATCGGCCTGACACCGCAAACTATCGCCCGTTTTCGTGA
ACGTTTTAATGCCCCCGTAGAAGTTCTGCATTCCGGCCTGAACGACAGCGAGCGTCTTTCGGCGTGGCTGAAAGCGAAAA
ATGGTGAGGCGGCGATTGTGATCGGCACCCGCTCCGCGCTGTTTACGCCGTTTAAAAATCTCGGTGTGATTGTCATTGAT
GAAGAGCACGACAGTTCCTACAAGCAGCAGGAAGGCTGGCGCTATCATGCCCGCGACCTGGCGGTTTATCGTGCGCACAG
CGAGCAAATCCCGATTATTCTTGGCTCTGCAACACCCGCACTGGAAACGTTATGCAACGTCCAGCAGAAAAAATACCGCC
TGCTGCGCCTGACGCGTCGGGCGGGGAATGCGCGTCCGGCAATTCAACACGTGCTGGATTTAAAAGGTCAGAAGGTGCAG
GCGGGGCTGGCTCCGGCGTTAATCACCCGTATGCGCCAGCATTTACAGGCCGACAACCAGGTTATCCTCTTTCTTAACCG
CCGTGGCTTTGCGCCTGCCTTGCTGTGCCACGACTGCGGCTGGATTGCCGAATGTCCACGTTGCGATCACTACTACACGC
TGCATCAGGCGCAACAGCATTTGCGCTGTCACCACTGTGACAGCCAACGCCCGGTGCCGCGCCAGTGCCCTTCCTGCGGT
TCCACGCACCTGGTACCCGTGGGGCTGGGCACCGAACAGCTTGAACAGACGCTCGCGCCGTTATTCCCTGGTGTGCCCAT
TTCTCGTATCGACCGCGATACCACCAGCCGCAAAGGAGCGCTGGAACAACAACTGGCGGAAGTGCATCGCGGCGGCGCGA
GGATCTTGATTGGCACGCAAATGCTGGCGAAAGGTCACCACTTTCCGGATGTGACGCTGGTAGCGTTACTGGATGTGGAC
GGCGCGCTGTTTTCTGCCGATTTTCGCTCGGCAGAGCGTTTTGCCCAGCTATACACCCAGGTAGCAGGCCGTGCCGGGCG
TGCGGGTAAACAGGGCGAAGTGGTGCTGCAAACGCATCATCCGGAACACCCGCTATTGCAAACGTTGCTCTATAAAGGCT
ACGACGCTTTTGCCGAACAGGCACTGGCTGAGCGACGCATGATGCAGCTGCCGCCGTGGACCAGCCATGTGATTGTGCGT
GCGGAAGATCATAACAATCAGCATGCGCCTCTGTTCCTGCAACAACTGCGTAATCTGATCCTCTCCAGCCCGCTGGCAGA
CGACAAATTGTGGGTTCTCGGTCCGGTTCCGGCACTGGCACCTAAACGTGGCGGTCGCTGGCGCTGGCAGATATTGTTGC
AGCACCCTTCCCGCGTGCGCTTGCAACACATCATTAGTGGTACGCTGGCGCTCATCAATACAATACCGGATTCCCGTAAG
GTGAAATGGGTGCTGGATGTTGATCCGATTGAGGGTTAA

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-101)

DEAD/DEAH box helicase (DEAD)

(203-362)

Helicase conserved C-terminal domain (Helicase_C)

(522-589)

Primosomal protein N C-terminal domain (PriA_C)

(632-729)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(445-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

44.776

100

0.451

Multiple sequence alignment