Detailed information    

insolico Bioinformatically predicted

Overview

Name   comL   Type   Machinery gene
Locus tag   ECRM12581_RS17175 Genome accession   NZ_CP007136
Coordinates   3338916..3339653 (+) Length   245 a.a.
NCBI ID   WP_000197686.1    Uniprot ID   P0AC03
Organism   Escherichia coli O145:H28 str. RM12581     
Function   DNA binding (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3333916..3344653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECRM12581_RS17160 (ECRM12581_16865) clpC 3334370..3336943 (-) 2574 WP_001235102.1 ATP-dependent chaperone ClpB Regulator
  ECRM12581_RS17165 (ECRM12581_16870) yfiH 3337073..3337804 (-) 732 WP_000040153.1 purine nucleoside phosphorylase YfiH -
  ECRM12581_RS17170 (ECRM12581_16875) rluD 3337801..3338781 (-) 981 WP_000079110.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  ECRM12581_RS17175 (ECRM12581_16880) comL 3338916..3339653 (+) 738 WP_000197686.1 outer membrane protein assembly factor BamD Machinery gene
  ECRM12581_RS17185 (ECRM12581_16885) raiA 3339924..3340265 (+) 342 WP_000178456.1 ribosome-associated translation inhibitor RaiA -
  ECRM12581_RS33720 pheL 3340369..3340416 (+) 48 WP_001386991.1 pheA operon leader peptide PheL -
  ECRM12581_RS17190 (ECRM12581_16890) pheA 3340515..3341675 (+) 1161 WP_000200122.1 bifunctional chorismate mutase/prephenate dehydratase -
  ECRM12581_RS17195 (ECRM12581_16895) tyrA 3341718..3342839 (-) 1122 WP_000225204.1 bifunctional chorismate mutase/prephenate dehydrogenase -
  ECRM12581_RS17200 (ECRM12581_16900) aroF 3342850..3343920 (-) 1071 WP_001168045.1 3-deoxy-7-phosphoheptulonate synthase AroF -
  ECRM12581_RS17205 (ECRM12581_16905) yfiL 3344130..3344495 (+) 366 WP_000976004.1 DUF2799 domain-containing protein -

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 27829.40 Da        Isoelectric Point: 6.4874

>NTDB_id=102439 ECRM12581_RS17175 WP_000197686.1 3338916..3339653(+) (comL) [Escherichia coli O145:H28 str. RM12581]
MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQDGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYY
KNADLPLAQAAIDRFIRLNPTHPNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAA
NSSNT

Nucleotide


Download         Length: 738 bp        

>NTDB_id=102439 ECRM12581_RS17175 WP_000197686.1 3338916..3339653(+) (comL) [Escherichia coli O145:H28 str. RM12581]
ATGACGCGCATGAAATATCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGT
ACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGC
AACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTAT
AAAAACGCCGATTTGCCGTTAGCGCAGGCTGCCATCGATCGTTTTATTCGCCTTAACCCGACCCATCCGAATATCGATTA
TGTCATGTACATGCGTGGCCTGACCAATATGGCGCTGGATGACAGTGCGCTGCAAGGGTTCTTTGGCGTTGACCGTAGCG
ATCGCGATCCTCAACATGCACGAGCTGCGTTTAGTGACTTTTCCAAACTGGTGCGCGGCTATCCGAACAGTCAGTACACC
ACCGATGCCACCAAACGTCTGGTATTCCTGAAAGATCGTCTGGCGAAATATGAATACTCCGTAGCCGAGTACTATACAGA
ACGTGGCGCATGGGTTGCCGTCGTTAACCGCGTAGAAGGCATGTTGCGCGACTACCCGGATACCCAGGCTACGCGTGATG
CGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCA
AACAGCAGCAATACATAA

Domains

Predicted by InterProScan.

Outer membrane lipoprotein (YfiO)

(28-236)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0AC03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

38.525

99.592

0.384
  comL Neisseria gonorrhoeae MS11

37.705

99.592

0.376

Multiple sequence alignment