Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB3G27_RS11280 Genome accession   NZ_CP162095
Coordinates   2241448..2242518 (-) Length   356 a.a.
NCBI ID   WP_249118252.1    Uniprot ID   -
Organism   Bacillus pumilus strain LBUM494     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2236448..2247518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G27_RS11230 (AB3G27_11230) sipW 2236541..2237113 (-) 573 WP_003217306.1 signal peptidase I SipW -
  AB3G27_RS11235 (AB3G27_11235) tapA 2237165..2237692 (-) 528 WP_137054980.1 amyloid fiber anchoring/assembly protein TapA -
  AB3G27_RS11240 (AB3G27_11240) - 2237992..2238270 (+) 279 WP_003217457.1 DUF3889 domain-containing protein -
  AB3G27_RS11245 (AB3G27_11245) - 2238304..2238498 (-) 195 WP_034620826.1 YqzE family protein -
  AB3G27_RS11250 (AB3G27_11250) comGG 2238558..2238941 (-) 384 WP_368196547.1 competence type IV pilus minor pilin ComGG -
  AB3G27_RS11255 (AB3G27_11255) comGF 2238938..2239438 (-) 501 WP_034620829.1 competence type IV pilus minor pilin ComGF -
  AB3G27_RS11260 (AB3G27_11260) comGE 2239377..2239694 (-) 318 WP_003217390.1 competence type IV pilus minor pilin ComGE -
  AB3G27_RS11265 (AB3G27_11265) comGD 2239678..2240124 (-) 447 WP_003217424.1 competence type IV pilus minor pilin ComGD -
  AB3G27_RS11270 (AB3G27_11270) comGC 2240117..2240410 (-) 294 WP_368196550.1 competence type IV pilus major pilin ComGC Machinery gene
  AB3G27_RS11275 (AB3G27_11275) comGB 2240427..2241467 (-) 1041 WP_368196552.1 competence type IV pilus assembly protein ComGB -
  AB3G27_RS11280 (AB3G27_11280) comGA 2241448..2242518 (-) 1071 WP_249118252.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3G27_RS11285 (AB3G27_11285) - 2242696..2243568 (-) 873 WP_003217438.1 STAS domain-containing protein -
  AB3G27_RS11295 (AB3G27_11295) - 2243792..2244172 (-) 381 WP_003217382.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  AB3G27_RS11300 (AB3G27_11300) - 2244385..2244627 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  AB3G27_RS11305 (AB3G27_11305) - 2244871..2245509 (-) 639 WP_003217463.1 MBL fold metallo-hydrolase -
  AB3G27_RS11310 (AB3G27_11310) - 2245667..2245840 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  AB3G27_RS11315 (AB3G27_11315) - 2246059..2246373 (-) 315 WP_003217504.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40361.76 Da        Isoelectric Point: 9.2487

>NTDB_id=1023994 AB3G27_RS11280 WP_249118252.1 2241448..2242518(-) (comGA) [Bacillus pumilus strain LBUM494]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRRGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLHRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1023994 AB3G27_RS11280 WP_249118252.1 2241448..2242518(-) (comGA) [Bacillus pumilus strain LBUM494]
TTGTATGGGATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTTCC
AAAGGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGAGAAAAACGGAGACCGCAAAATGGGTCATTAGCT
GTGATGCTAAGAAGGGGTCAGGTGTTTATTCGGATGTCTACCCTACCAACTGTAAATGATGAGAGCTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACACCTGTCACTATTTCCGAAAGCATCCACCAGGTTATTATCCTTTTTGA
ATCATTCGCATGGGCTCATTTTATTTACCGGTCCAACCAATTCAGGGAAAACAACAACCCTTTATTCATTGATCCAGTTT
GCAAAAAAGAATTTCAACAGAAATATTATTACACTTGAAGATCCTGTGGAAACGAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGTATCACATATGCGGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCTCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACACTCCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTGGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGCCAGCTTTACTGTAAATTAAATCGAC
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCATAGATTAATTCGTAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.407

99.438

0.64

Multiple sequence alignment