Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   AB3F25_RS01925 Genome accession   NZ_CP162010
Coordinates   380633..381172 (+) Length   179 a.a.
NCBI ID   WP_373604306.1    Uniprot ID   -
Organism   Aggregatibacter sp. HMT-949 strain W10649     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 375633..386172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3F25_RS01905 (AB3F25_01900) - 376295..377212 (-) 918 WP_373603842.1 ATP-binding cassette domain-containing protein -
  AB3F25_RS01910 (AB3F25_01905) - 377212..378093 (-) 882 WP_373603843.1 metal ABC transporter substrate-binding protein -
  AB3F25_RS01915 (AB3F25_01910) - 378336..379217 (-) 882 WP_373603844.1 D-alanyl-D-alanine carboxypeptidase family protein -
  AB3F25_RS01920 (AB3F25_01915) - 379451..380587 (+) 1137 WP_373603845.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  AB3F25_RS01925 (AB3F25_01920) pilF/pilF2 380633..381172 (+) 540 WP_373604306.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AB3F25_RS01930 (AB3F25_01925) - 381241..382248 (+) 1008 WP_373603846.1 RodZ domain-containing protein -
  AB3F25_RS01935 (AB3F25_01930) ispG 382260..383366 (+) 1107 WP_373603847.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AB3F25_RS01940 (AB3F25_01935) hisS 383376..384647 (+) 1272 WP_373603848.1 histidine--tRNA ligase -
  AB3F25_RS01945 (AB3F25_01940) - 384659..385273 (+) 615 WP_373603849.1 YfgM family protein -
  AB3F25_RS01950 (AB3F25_01945) - 385362..385748 (+) 387 WP_373603850.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20427.00 Da        Isoelectric Point: 8.2399

>NTDB_id=1023478 AB3F25_RS01925 WP_373604306.1 380633..381172(+) (pilF/pilF2) [Aggregatibacter sp. HMT-949 strain W10649]
MKVIRFVGAIIFPLLFSACVSQNDTVAFNKQRAAKARVELALGYLQQNNLPQAKQNLDKALEHDKNYYLVYSALAHFYQL
QGDASAAHEAYQQALKLDPKQGDTHNNFGAFLCGRGEFVQAYEQFEAALSSPNYYRQADTYENIALCAQAENRRELYQQA
FDKLRQIDVHRAEKLNRAK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1023478 AB3F25_RS01925 WP_373604306.1 380633..381172(+) (pilF/pilF2) [Aggregatibacter sp. HMT-949 strain W10649]
ATCAAGGTTATTCGATTTGTAGGAGCGATCATTTTCCCTTTGCTTTTCTCTGCTTGTGTTTCACAAAATGACACCGTTGC
TTTTAATAAACAACGTGCGGCAAAGGCGCGTGTAGAACTCGCTCTCGGTTATCTTCAACAAAATAATCTACCGCAAGCTA
AACAGAATTTGGATAAAGCCTTAGAGCATGACAAAAATTATTATCTAGTGTATTCCGCACTGGCACATTTTTATCAATTG
CAGGGGGATGCGAGCGCCGCGCACGAAGCTTATCAACAAGCGCTTAAATTAGACCCAAAACAAGGCGATACGCATAATAA
TTTCGGTGCATTTTTATGTGGGCGAGGCGAGTTTGTGCAGGCCTATGAACAATTTGAGGCAGCGTTGTCTTCGCCGAATT
ATTATCGTCAAGCGGATACTTATGAAAATATAGCGCTTTGCGCACAGGCGGAAAATCGGCGGGAACTGTATCAACAGGCT
TTTGATAAACTGCGGCAAATCGATGTTCATCGCGCGGAAAAGCTGAATCGAGCCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

61.667

100

0.62
  pilF Glaesserella parasuis strain SC1401

48.901

100

0.497

Multiple sequence alignment