Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   DQM95_RS00770 Genome accession   NZ_LS483397
Coordinates   124648..125682 (+) Length   344 a.a.
NCBI ID   WP_408646205.1    Uniprot ID   -
Organism   Streptococcus uberis strain NCTC3858     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119648..130682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM95_RS00760 (NCTC3858_00155) - 123336..123701 (+) 366 WP_041817508.1 DUF1033 family protein -
  DQM95_RS00765 (NCTC3858_00156) comGA/cglA 123774..124715 (+) 942 WP_037592994.1 competence type IV pilus ATPase ComGA Machinery gene
  DQM95_RS00770 (NCTC3858_00157) comGB 124648..125682 (+) 1035 WP_408646205.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQM95_RS00780 (NCTC3858_00159) - 125763..127208 (-) 1446 WP_037592992.1 recombinase family protein -
  DQM95_RS00785 (NCTC3858_00160) - 127358..127807 (-) 450 WP_037592991.1 Ltp family lipoprotein -
  DQM95_RS00790 (NCTC3858_00161) - 127860..128237 (-) 378 WP_037593023.1 ImmA/IrrE family metallo-endopeptidase -
  DQM95_RS00795 (NCTC3858_00162) - 128221..128583 (-) 363 WP_037592990.1 helix-turn-helix domain-containing protein -
  DQM95_RS00800 (NCTC3858_00163) - 128774..128974 (+) 201 WP_037592989.1 helix-turn-helix transcriptional regulator -
  DQM95_RS00805 (NCTC3858_00164) - 129021..129746 (+) 726 WP_037592987.1 phage antirepressor KilAC domain-containing protein -
  DQM95_RS00810 (NCTC3858_00165) - 129758..130099 (+) 342 WP_037592986.1 hypothetical protein -
  DQM95_RS00815 (NCTC3858_00166) - 130100..130312 (-) 213 WP_037592984.1 hypothetical protein -
  DQM95_RS00820 - 130462..130647 (+) 186 WP_037592983.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39861.54 Da        Isoelectric Point: 9.5535

>NTDB_id=1022752 DQM95_RS00770 WP_408646205.1 124648..125682(+) (comGB) [Streptococcus uberis strain NCTC3858]
MSNFLNKDISITKKPSWKKLKAKQQYQLIKLLHNLSLSGFNLNEMVSFLEKAHLLEDHFVQRMKIDLMNGRDISEMMAHL
GFSDALVTQLSLAEVHGNTTNCLAKIEDYMAQSNRIRQKTIEVITYPMVLMTFLIVILMGLRHYLLPQIGEENQLTHFLN
LFPTVLLLTFFAMIAVVIFLRLCWRKKSQLKQLLVYSKIPLLSAVLQLYLTAYYAREWGNLIAQGVELATILQLMQKEKS
VLMREMGSTMEKALLEGKSFDQSVGIFPFFKRELSLMIEYGEIKGKLGQELDIYAQVSWENYFRKLRQMTQWLQPLIFIL
VAIIIVMIYAAMLLPMYQTIGGEI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1022752 DQM95_RS00770 WP_408646205.1 124648..125682(+) (comGB) [Streptococcus uberis strain NCTC3858]
TTGAGCAACTTCTTAAACAAGGATATATCGATAACGAAAAAGCCAAGTTGGAAAAAATTAAAGGCTAAACAACAATACCA
GCTGATCAAATTATTACATAATCTTAGTCTAAGTGGGTTTAACTTGAACGAAATGGTATCTTTTCTTGAGAAGGCACATC
TCTTAGAGGATCATTTTGTTCAAAGAATGAAAATAGATTTAATGAATGGACGAGATATTTCGGAAATGATGGCCCATCTT
GGATTCTCAGATGCATTGGTAACACAGCTAAGTTTAGCTGAAGTCCATGGGAACACCACCAACTGTTTAGCAAAGATAGA
AGATTATATGGCTCAATCCAATCGGATTAGACAAAAAACCATTGAAGTCATTACTTATCCTATGGTCTTGATGACCTTCT
TAATCGTGATTTTGATGGGCTTACGCCATTATTTACTTCCGCAAATAGGAGAGGAAAACCAGCTAACACATTTTCTAAAT
TTATTTCCGACAGTATTGCTTTTGACTTTCTTTGCTATGATTGCGGTAGTAATCTTCCTCAGACTCTGCTGGCGAAAAAA
ATCCCAGTTAAAACAATTATTAGTGTATAGTAAAATCCCTCTTTTATCAGCTGTTTTACAATTGTATCTAACTGCCTATT
ATGCGAGAGAGTGGGGCAATCTTATTGCACAAGGTGTTGAACTAGCAACTATTTTGCAACTGATGCAAAAGGAAAAATCC
GTATTGATGCGGGAAATGGGTTCAACAATGGAAAAAGCTTTGTTGGAAGGGAAATCTTTTGACCAGTCTGTGGGCATCTT
CCCATTTTTTAAAAGGGAATTGAGCTTAATGATTGAATATGGAGAAATCAAAGGAAAACTCGGTCAAGAGTTAGACATTT
ATGCACAAGTCAGTTGGGAAAACTATTTTAGAAAGTTACGACAGATGACTCAATGGTTACAACCCCTCATTTTCATACTT
GTAGCCATTATAATCGTTATGATTTATGCGGCAATGTTATTGCCAATGTATCAAACCATCGGAGGTGAAATATAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(31-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Streptococcus mutans UA159

53.254

98.256

0.523
  comGB Streptococcus mutans UA140

53.254

98.256

0.523
  comGB Streptococcus gordonii str. Challis substr. CH1

47.353

98.837

0.468
  comGB/cglB Streptococcus mitis NCTC 12261

47.305

97.093

0.459
  comGB Lactococcus lactis subsp. cremoris KW2

45.161

99.128

0.448
  comGB/cglB Streptococcus mitis SK321

45.946

96.802

0.445
  comGB/cglB Streptococcus pneumoniae Rx1

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae D39

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae R6

44.745

96.802

0.433
  comGB/cglB Streptococcus pneumoniae TIGR4

44.745

96.802

0.433

Multiple sequence alignment