Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ABU640_RS01355 Genome accession   NZ_CP161854
Coordinates   295667..296683 (+) Length   338 a.a.
NCBI ID   WP_032481153.1    Uniprot ID   -
Organism   Vibrio cholerae strain C3616     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 290667..301683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABU640_RS01340 (ABU640_01340) - 291208..291939 (-) 732 WP_000495226.1 glutathione peroxidase -
  ABU640_RS01345 (ABU640_01345) oxyR 292079..292972 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  ABU640_RS01350 (ABU640_01350) - 293047..295524 (-) 2478 WP_032481152.1 penicillin-binding protein 1A -
  ABU640_RS01355 (ABU640_01355) pilM 295667..296683 (+) 1017 WP_032481153.1 type IV pilus assembly protein PilM Machinery gene
  ABU640_RS01360 (ABU640_01360) pilN 296673..297257 (+) 585 WP_161418895.1 PilN domain-containing protein Machinery gene
  ABU640_RS01365 (ABU640_01365) pilO 297250..297840 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  ABU640_RS01370 (ABU640_01370) pilP 297830..298348 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  ABU640_RS01375 (ABU640_01375) pilQ 298374..300089 (+) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  ABU640_RS01380 (ABU640_01380) aroK 300276..300800 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37735.49 Da        Isoelectric Point: 7.3328

>NTDB_id=1022752 ABU640_RS01355 WP_032481153.1 295667..296683(+) (pilM) [Vibrio cholerae strain C3616]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1022752 ABU640_RS01355 WP_032481153.1 295667..296683(+) (pilM) [Vibrio cholerae strain C3616]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGTGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTATATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTTAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.817

100

0.488

Multiple sequence alignment