Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ABZM97_RS05900 Genome accession   NZ_CP160797
Coordinates   1189215..1192919 (+) Length   1234 a.a.
NCBI ID   WP_367387301.1    Uniprot ID   -
Organism   Bacillus vallismortis strain BL-01     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1184215..1197919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZM97_RS05890 (ABZM97_05890) - 1185079..1185543 (-) 465 WP_253268993.1 ferritin family protein -
  ABZM97_RS05895 (ABZM97_05895) addB 1185728..1189228 (+) 3501 WP_253268994.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ABZM97_RS05900 (ABZM97_05900) addA 1189215..1192919 (+) 3705 WP_367387301.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ABZM97_RS05905 (ABZM97_05905) sbcD 1192994..1194169 (+) 1176 WP_253268996.1 exonuclease subunit SbcD -
  ABZM97_RS05910 (ABZM97_05910) sbcC 1194166..1197558 (+) 3393 WP_202328475.1 exonuclease subunit SbcC -
  ABZM97_RS05915 (ABZM97_05915) hlpB 1197575..1197871 (+) 297 WP_148964229.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141242.81 Da        Isoelectric Point: 5.3589

>NTDB_id=1022011 ABZM97_RS05900 WP_367387301.1 1189215..1192919(+) (addA) [Bacillus vallismortis strain BL-01]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRMADQAEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLHGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQGDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNSAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENETGKREPSEAARFYQEQ
FREVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RTDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETKTELLLIDNTEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRSFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQMRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQPDWLLPEFDRYQAKTYLDFIGPALARHRDLENSAENGAAAHADISGHPARFAVRMIHSYD
LLDDDLAERMEEKSDRLEAIRKGEPVPGSFAFDKKAREQLSWTYPHQEVTQIRTKQSVSEIKRKREYEDEYTGRAPVKPA
DGSILYRRPAFMMKQGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVHRLYEKELLTEEQKAAIDIEEIVRFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETENGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1022011 ABZM97_RS05900 WP_367387301.1 1189215..1192919(+) (addA) [Bacillus vallismortis strain BL-01]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCCGGAAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
TTGGAAAAAGAGCTTGTAAAACGCCCCGGATCACTGCATATCAGGCGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTAATCGATCTTGATCCGGGCTTTCGGATGGCTG
ATCAGGCAGAAGGCGAGCTGCTTGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGCGAGAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCATGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCGAACCCGGAGGCATGGCTGGAGAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGCGCCATCG
AAGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCCATGGTGCTTCACGGAGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACAAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGGTGATTTCACTGAACTTTATAAACGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAATAGCGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGATTACTTCACGAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGACATGAAGCCTGTCATTGAAACGCTTGTACA
GCTTGTCATCAGCTACGGAAAACGATTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTCTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCAGAGAATGAAACAGGTAAACGCGAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCGCGAGGTGCTCGTTGACGAATACCAGGATACCAACCTCGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCTGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGATTTACAGAGAGCGGAGAAGGTACGGGACGGAAAATTGACTTGAATAAAAATTTCCGAAGC
CGGACTGATATTTTAGACAGCACGAACTTTTTATTTAAACAGCTAATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATGAGACGAAAACGGAGCTGCTGCTGATCGACAACACAG
AAGAGACGGATGCAGGTGAGGAAGCAGAAGAGCTGGAAACCGTGCAGTTCGAAGCGAAAGCGATCGCCAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTCCGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTTTGAAGCGGTCGAAGTCGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGTTCCCCGATTGTCGGGGCGGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCGGCGGGTGACCGTAACGACGAGCTTTATCAAAAGCTGCAAACAT
TCTACGGACATCTGCAAAAATGGCGTTCGTTTTCGAAAAACCACTCCGTATCCGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCGGCGGGTCTCGGCCGGAACTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGATTTGGCACGAAGTTTATCCATCCGCAAATGCGCATCAGCTACCCGACTCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGAGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGAGGGCGAAAGAAAAATTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAACCTGACTGGCTTCTGCCCGA
ATTTGACCGCTATCAGGCGAAAACGTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGATTTGGAGAATTCAG
CTGAAAACGGTGCGGCAGCGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTTGATGATGATTTGGCAGAAAGAATGGAAGAAAAAAGCGATCGCCTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCTCGTTTGCATTTGATAAAAAAGCCCGCGAGCAGCTGAGCTGGACATACCCGCATCAAGAGGTCACACAAATTCGAA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACACCGGCAGGGCTCCCGTTAAACCGGCT
GACGGAAGCATTCTGTACAGGCGCCCCGCTTTTATGATGAAACAGGGCCTGACAGCGGCGGAGAAAGGGACAGCCATGCA
TACAGTGATGCAGCATATCCCGCTGACACATGTGCCGACGATAGAAGAAGCGGAGCAGACGGTTCACAGGCTTTATGAAA
AGGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTGCGATTTTTCCATACGGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAAGAAATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAAAACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCGGTTGAGCAAATTGCGAAAACAAAGGTAAAAGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATACTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

95.786

100

0.958

Multiple sequence alignment