Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   U2F28_RS08340 Genome accession   NZ_CP160635
Coordinates   1697003..1697689 (-) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   A0A7U7EYP5
Organism   Staphylococcus aureus strain BSN44     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1692003..1702689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2F28_RS08320 (U2F28_008320) rpsT 1692917..1693168 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  U2F28_RS08325 (U2F28_008325) holA 1693213..1694187 (-) 975 WP_001282563.1 DNA polymerase III subunit delta -
  U2F28_RS08330 (U2F28_008330) comEC 1694244..1696445 (-) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  U2F28_RS08335 (U2F28_008335) - 1696450..1696911 (-) 462 WP_000439693.1 ComE operon protein 2 -
  U2F28_RS08340 (U2F28_008340) comEA 1697003..1697689 (-) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  U2F28_RS08345 (U2F28_008345) - 1697729..1698445 (-) 717 WP_000084830.1 class I SAM-dependent methyltransferase -
  U2F28_RS08350 (U2F28_008350) rsfS 1698448..1698801 (-) 354 WP_001088020.1 ribosome silencing factor -
  U2F28_RS08355 (U2F28_008355) yqeK 1698802..1699386 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  U2F28_RS08360 (U2F28_008360) nadD 1699376..1699945 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  U2F28_RS08365 (U2F28_008365) yhbY 1699948..1700238 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  U2F28_RS08370 (U2F28_008370) aroE 1700242..1701048 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  U2F28_RS08375 (U2F28_008375) yqeH 1701062..1702162 (-) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=1021810 U2F28_RS08340 WP_001802022.1 1697003..1697689(-) (comEA) [Staphylococcus aureus strain BSN44]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1021810 U2F28_RS08340 WP_001802022.1 1697003..1697689(-) (comEA) [Staphylococcus aureus strain BSN44]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EYP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

100

1
  comEA Staphylococcus aureus MW2

97.368

100

0.974

Multiple sequence alignment