Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   U2F28_RS08090 Genome accession   NZ_CP160635
Coordinates   1649615..1650112 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BSN44     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644615..1655112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U2F28_RS08065 (U2F28_008065) gcvPB 1644777..1646249 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  U2F28_RS08070 (U2F28_008070) gcvPA 1646242..1647588 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  U2F28_RS08075 (U2F28_008075) gcvT 1647608..1648699 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  U2F28_RS08080 (U2F28_008080) - 1648858..1649382 (-) 525 WP_001015120.1 shikimate kinase -
  U2F28_RS08085 (U2F28_008085) - 1649372..1649518 (-) 147 WP_001792109.1 hypothetical protein -
  U2F28_RS08090 (U2F28_008090) comGF 1649615..1650112 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  U2F28_RS08095 (U2F28_008095) comGE 1650030..1650329 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  U2F28_RS08100 (U2F28_008100) comGD 1650316..1650762 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  U2F28_RS08105 (U2F28_008105) comGC 1650740..1651051 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  U2F28_RS08110 (U2F28_008110) comGB 1651065..1652135 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  U2F28_RS08115 (U2F28_008115) comGA 1652107..1653081 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  U2F28_RS08120 (U2F28_008120) - 1653133..1653756 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  U2F28_RS08125 (U2F28_008125) - 1653753..1654082 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  U2F28_RS08130 (U2F28_008130) - 1654082..1655068 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=1021802 U2F28_RS08090 WP_001788897.1 1649615..1650112(-) (comGF) [Staphylococcus aureus strain BSN44]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1021802 U2F28_RS08090 WP_001788897.1 1649615..1650112(-) (comGF) [Staphylococcus aureus strain BSN44]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment