Detailed information NTG1021645

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V5222_RS21785
• Genome accession: NZ_CP160508
• Coordinates: 4395456..4396256 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 7Gaza2023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4390456..4401256

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5222_RS21750	gspH	4390612..4391127 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
V5222_RS21755	gspI	4391124..4391489 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
V5222_RS21760	gspJ	4391486..4392082 (+)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
V5222_RS21765	gspK	4392079..4393065 (+)	987	WP_077254241.1	type II secretion system minor pseudopilin GspK	-
V5222_RS21770	gspL	4393052..4394245 (+)	1194	WP_032438747.1	type II secretion system protein GspL	-
V5222_RS21775	-	4394238..4394723 (+)	486	WP_004151943.1	type II secretion system protein M	-
V5222_RS21780	-	4394713..4395459 (+)	747	WP_032438748.1	type II secretion system protein N	-
V5222_RS21785	pilD	4395456..4396256 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
V5222_RS21790	hrpB	4396304..4398733 (-)	2430	WP_032437605.1	ATP-dependent helicase HrpB	-
V5222_RS21795	thpR	4398806..4399341 (+)	536	Protein_4273	RNA 2',3'-cyclic phosphodiesterase	-
V5222_RS21800	sfsA	4399341..4400057 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
V5222_RS21805	dksA	4400220..4400675 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=1021645 V5222_RS21785 WP_004213441.1 4395456..4396256(+) (pilD) [Klebsiella pneumoniae strain 7Gaza2023]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1021645 V5222_RS21785 WP_004213441.1 4395456..4396256(+) (pilD) [Klebsiella pneumoniae strain 7Gaza2023]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425