Detailed information NTG1021606

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V5218_RS04460
• Genome accession: NZ_CP160503
• Coordinates: 952878..953678 (-)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain 6Gaza2023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 947878..958678

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5218_RS04440	dksA	948458..948913 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
V5218_RS04445	sfsA	949076..949792 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
V5218_RS04450	thpR	949792..950328 (-)	537	WP_032416682.1	RNA 2',3'-cyclic phosphodiesterase	-
V5218_RS04455	hrpB	950401..952830 (+)	2430	WP_032437605.1	ATP-dependent helicase HrpB	-
V5218_RS04460	pilD	952878..953678 (-)	801	WP_004213441.1	prepilin peptidase	Machinery gene
V5218_RS04465	-	953675..954421 (-)	747	WP_032438748.1	type II secretion system protein N	-
V5218_RS04470	-	954411..954896 (-)	486	WP_004151943.1	type II secretion system protein M	-
V5218_RS04475	gspL	954889..956082 (-)	1194	WP_032438747.1	type II secretion system protein GspL	-
V5218_RS04480	gspK	956069..957055 (-)	987	WP_077254241.1	type II secretion system minor pseudopilin GspK	-
V5218_RS04485	gspJ	957052..957648 (-)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
V5218_RS04490	gspI	957645..958010 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
V5218_RS04495	gspH	958007..958522 (-)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=1021606 V5218_RS04460 WP_004213441.1 952878..953678(-) (pilD) [Klebsiella pneumoniae strain 6Gaza2023]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=1021606 V5218_RS04460 WP_004213441.1 952878..953678(-) (pilD) [Klebsiella pneumoniae strain 6Gaza2023]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425