Detailed information NTG1021597

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: V5226_RS22070
• Genome accession: NZ_CP160499
• Coordinates: 4452020..4452820 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain 51Gaza2023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4447020..4457820

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V5226_RS22035	gspH	4447176..4447691 (+)	516	WP_032422016.1	type II secretion system minor pseudopilin GspH	-
V5226_RS22040	gspI	4447688..4448053 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
V5226_RS22045	gspJ	4448050..4448646 (+)	597	WP_032433846.1	type II secretion system minor pseudopilin GspJ	-
V5226_RS22050	gspK	4448643..4449629 (+)	987	WP_077255453.1	type II secretion system minor pseudopilin GspK	-
V5226_RS22055	gspL	4449616..4450809 (+)	1194	WP_032416685.1	type II secretion system protein GspL	-
V5226_RS22060	-	4450802..4451287 (+)	486	WP_004145896.1	type II secretion system protein M	-
V5226_RS22065	-	4451277..4452023 (+)	747	WP_004145897.1	type II secretion system protein N	-
V5226_RS22070	pilD	4452020..4452820 (+)	801	WP_004191960.1	prepilin peptidase	Machinery gene
V5226_RS22075	hrpB	4452868..4455297 (-)	2430	WP_064472269.1	ATP-dependent helicase HrpB	-
V5226_RS22080	thpR	4455370..4455906 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
V5226_RS22085	sfsA	4455906..4456622 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
V5226_RS22090	dksA	4456785..4457240 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=1021597 V5226_RS22070 WP_004191960.1 4452020..4452820(+) (pilD) [Klebsiella pneumoniae strain 51Gaza2023]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=1021597 V5226_RS22070 WP_004191960.1 4452020..4452820(+) (pilD) [Klebsiella pneumoniae strain 51Gaza2023]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421